Document Detail


c-Jun ARE targets mRNA deadenylation by an EDEN-BP (embryo deadenylation element-binding protein)-dependent pathway.
MedLine Citation:
PMID:  11707455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mammalian cells, certain mRNAs encoding cytokines or proto-oncogenes are especially unstable, because of the presence of a particular sequence element in their 3'-untranslated region named ARE (A/U-rich element). AREs cause this instability by provoking the rapid shortening of the poly(A) tail of the mRNA. The deadenylation of mRNAs mediated by AREs containing repeats of the AUUUA motif (class I/II AREs) is conserved in Xenopus embryos. Here, we first extend these observations by showing that c-Jun ARE, a representative of class III (non-AUUUA) AREs, also provokes the deadenylation of a reporter RNA in Xenopus embryos. Next, by immunodepletion and immunoneutralization experiments, we show that, in Xenopus, the rapid deadenylation of RNAs that contain the c-Jun ARE, but not an AUUUA ARE, requires EDEN-BP. This RNA-binding protein was previously shown to provoke the rapid deadenylation of certain Xenopus maternal RNAs. Finally, we show that CUG-BP, the human homologue of EDEN-BP, specifically binds to c-Jun ARE. Together, these results identify CUG-BP as a plausible deadenylation factor responsible for the post-transcriptional control of c-Jun proto-oncogene mRNA in mammalian cells.
Authors:
Luc Paillard; Vincent Legagneux; Dominique Maniey; H Beverley Osborne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-11-13
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  277     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-28     Completed Date:  2002-02-28     Revised Date:  2010-09-16    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3232-5     Citation Subset:  IM    
Affiliation:
CNRS UMR 6061, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, 35043 Rennes Cedex, France. Luc.Paillard@univ-rennes1.fr
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MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions / genetics
Animals
Cloning, Molecular
DNA Primers
Embryo, Nonmammalian
Humans
Oligodeoxyribonucleotides, Antisense
Open Reading Frames
Proto-Oncogene Proteins c-jun / genetics*
RNA Processing, Post-Transcriptional
RNA, Messenger / metabolism*
RNA-Binding Proteins / genetics,  metabolism*
Restriction Mapping
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleoproteins / genetics,  metabolism
Transcription, Genetic
Xenopus Proteins*
Xenopus laevis / embryology
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/CELF1 protein, human; 0/DNA Primers; 0/EDEN-specific RNA-binding protein, Xenopus; 0/Oligodeoxyribonucleotides, Antisense; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/RNA-Binding Proteins; 0/Ribonucleoproteins; 0/Xenopus Proteins

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