| c-Jun/AP-1 pathway-mediated cyclin D1 expression participates in low dose arsenite-induced transformation in mouse epidermal JB6 Cl41 cells. | |
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MedLine Citation:
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PMID: 19059425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arsenic is a well-documented human carcinogen associated with skin carcinogenesis. Our previous work reveals that arsenite exposure is able to induce cell transformation in mouse epidermal cell JB6 Cl41 through the activation of ERK, rather than JNK pathway. Our current studies further evaluate downstream pathway in low dose arsenite-induced cell transformation in JB6 Cl41 cells. Our results showed that treatment of cells with low dose arsenite induced activation of c-Jun/AP-1 pathway, and ectopic expression of dominant negative mutant of c-Jun (TAM67) blocked arsenite-induced transformation. Furthermore, our data indicated that cyclin D1 was an important downstream molecule involved in c-Jun/AP-1-mediated cell transformation upon low dose arsenite exposure, because inhibition of cyclin D1 expression by its specific siRNA in the JB6 Cl41 cells resulted in impairment of anchorage-independent growth of cells induced by low dose arsenite. Collectively, our results demonstrate that c-Jun/AP-1-mediated cyclin D1 expression is at least one of the key events implicated in cell transformation upon low dose arsenite exposure. |
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Authors:
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Dongyun Zhang; Jingxia Li; Jimin Gao; Chuanshu Huang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-11-14 |
Journal Detail:
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Title: Toxicology and applied pharmacology Volume: 235 ISSN: 1096-0333 ISO Abbreviation: Toxicol. Appl. Pharmacol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-09 Completed Date: 2009-03-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0416575 Medline TA: Toxicol Appl Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 18-24 Citation Subset: IM |
Affiliation:
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Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arsenites / administration & dosage, metabolism*, toxicity* Cell Line Cyclin D1 / genetics, metabolism* Dose-Response Relationship, Drug Environmental Pollutants / metabolism, toxicity Epidermis / cytology* JNK Mitogen-Activated Protein Kinases / metabolism* Mice |
| Grant Support | |
ID/Acronym/Agency:
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CA094964/CA/NCI NIH HHS; CA112557/CA/NCI NIH HHS; ES000260/ES/NIEHS NIH HHS; ES012451/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arsenites; 0/Ccnd1 protein, mouse; 0/Environmental Pollutants; 136601-57-5/Cyclin D1; 15502-74-6/arsenite; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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