Document Detail

The bystander effect is a novel mechanism of UVA-induced melanogenesis.
MedLine Citation:
PMID:  22091933     Owner:  NLM     Status:  Publisher    
We successfully identified the bystander effect in B16 murine melanoma cells exposed to UVA irradiation. The effect was identified based on melanogenesis following the medium transfer of the B16 cells, which had been cultured for 24 hours after being exposed to UVA irradiation, to non-irradiated cells (bystander cells). Our confirmation study of the functional mechanism of bystander cells confirmed the reduced levels of mitochondrial membrane potential 1-3 hours after the medium transfer. In addition, we observed increased levels of intracellular oxidation after 12 hours, and the generation of melanin radicals, including long-lived radicals, 24 hours after medium transfer. Further analysis of bystander factors revealed that the administration of EGTA treatment at the time of medium transfer led to an inhibition of melanogenesis and to neutralization of the mitochondrial membrane potential level, as well as to the restoration of intracellular oxidation levels to those of controls. The results demonstrated that the UVA irradiation bystander effect in B16 cells, as indicated by melanogenesis, was induced by the increase in intracellular oxidation due to the mitochondrial activity of calcium ions, which were among the bystander factors involved in the increase.
Hideki Nishiura; Jun Kumagai; Genro Kashino; Takuya Okada; Keizo Tano; Masami Watanabe
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-17
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  -     ISSN:  1751-1097     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Photochemistry and Photobiology © 2011 American Society for Photobiology.
Laboratory of Radiation Biology, Division of Radiation Life Science, Department of Radiation Life Science and Radiation Medical Science, Kyoto University Research Reactor Institute, 2-1010 Asashiro-nishi, Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan Nihon Kolmar Co., Ltd. Kashiwara Laboratory, 2-16 Enmyo-cho, Kashiwara-city, Osaka 582-0027, Japan Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603, Japan Advanced Molecular Imaging Center, School of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu city, Oita, 879-5593, Japan.
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