Document Detail


NIP6;1 is a boric acid channel for preferential transport of boron to growing shoot tissues in Arabidopsis.
MedLine Citation:
PMID:  18952773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Boron (B) in soil is taken up by roots through NIP5;1, a boric acid channel, and is loaded into the xylem by BOR1, a borate exporter. Here, the function of Arabidopsis thaliana NIP6;1, the most similar gene to NIP5;1, was studied. NIP6;1 facilitates the rapid permeation of boric acid across the membrane but is completely impermeable to water. NIP6;1 transcript accumulation is elevated in response to B deprivation in shoots but not in roots. NIP6;1 promoter-beta-glucuronidase is predominantly expressed in nodal regions of shoots, especially the phloem region of vascular tissues. Three independently identified T-DNA insertion lines for the NIP6;1 gene exhibited reduced expansion of young rosette leaves only under low-B conditions. B concentrations are reduced in young rosette leaves but not in the old leaves of these mutants. Taken together, these data strongly suggest that NIP6;1 is a boric acid channel required for proper distribution of boric acid, particularly among young developing shoot tissues. We propose that NIP6;1 is involved in xylem-phloem transfer of boric acid at the nodal regions and that the water-tight property of NIP6;1 is important for this function. It is proposed that during evolution, NIP5;1 and NIP6;1 were diversified in terms of both the specificity of their expression in plant tissues and their water permeation properties, while maintaining their ability to be induced under low B and their boric acid transport activities.
Authors:
Mayuki Tanaka; Ian S Wallace; Junpei Takano; Daniel M Roberts; Toru Fujiwara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-10-24
Journal Detail:
Title:  The Plant cell     Volume:  20     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-12-05     Completed Date:  2009-02-19     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2860-75     Citation Subset:  IM    
Affiliation:
Graduate School of Agricultural and Life Science, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / genetics,  metabolism*
Arabidopsis Proteins / analysis,  genetics,  physiology*
Biological Transport
Boron / metabolism*
Cell Membrane / metabolism
Glucuronidase / analysis
Membrane Transport Proteins / analysis,  genetics,  physiology*
Mutagenesis, Insertional
Oocytes / metabolism
Permeability
Phloem / metabolism
Plant Leaves / metabolism
Plant Roots / metabolism
Plant Shoots / metabolism
Promoter Regions, Genetic
RNA, Messenger / metabolism
Recombinant Fusion Proteins / analysis
Water / metabolism
Xenopus
Xylem / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Membrane Transport Proteins; 0/NIP6;1 protein, Arabidopsis; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 7440-42-8/Boron; 7732-18-5/Water; EC 3.2.1.31/Glucuronidase
Comments/Corrections

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