Document Detail


The blood group ABO gene transcript is down-regulated in human bladder tumors and growth-stimulated urothelial cell lines.
MedLine Citation:
PMID:  8640757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular mechanism that in human bladder tumors leads to the loss of blood group ABO glycosyltransferase activity and, thereby, the loss of ABO antigens was investigated. In 15 tumors and 3 normal biopsies from blood group AB individuals and 7 tumors and 3 normal biopsies from blood group O individuals, mRNA was detected by a reverse transcription PCR (RT-PCR) assay, and the ABO blood group structure was determined by immunohistology. The RT-PCR spanned several introns in the ABO gene to exclude DNA contamination, and the RT-PCR product was shown to reflect the ABO gene message by dideoxy sequencing. The ABO mRNA was present in normal urothelium and low-grade tumors but disappeared from high grade tumors. This correlation to tumor grade was significant (P<0.04). Immunohistochemistry with monoclonal anti-blood group antibodies showed a complete correlation between the presence of mRNA and the presence of AB carbohydrate structures on cell surfaces. In two urothelial cell lines, genotyped as A/- and A/A, growth stimulation with the cholera toxin B subunit led to a total loss of ABO mRNA, and epidermal growth factor stimulation had an identical effect on one of the cell lines. We conclude that the ABO glycosylation in normal and malignant urothelium is regulated at the mRNA level, and that a mechanism associated with cell proliferation may trigger down-regulation of ABO mRNA.
Authors:
T F Orntoft; P Meldgaard; B Pedersen; H Wolf
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  56     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-07-15     Completed Date:  1996-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1031-36     Citation Subset:  IM    
Affiliation:
Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, Denmark.
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MeSH Terms
Descriptor/Qualifier:
ABO Blood-Group System / genetics*,  metabolism
Base Sequence
Cell Division
Cells, Cultured
Down-Regulation
Humans
Immunohistochemistry
Karyotyping
Molecular Sequence Data
Polymerase Chain Reaction
Transcription, Genetic
Urinary Bladder Neoplasms / blood,  genetics*
Urinary Tract / pathology*
Chemical
Reg. No./Substance:
0/ABO Blood-Group System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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