Document Detail


The blood flow in pancreatico-duodenal grafts in rats: inhibition of nitric oxide synthase preferentially decreases islet blood flow.
MedLine Citation:
PMID:  7539767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study normoglycemic inbred Wistar-Furth rats were implanted with a syngeneic pancreatico-duodenal graft, i.e. a denervated pancreas. The blood flow to the intact native pancreas and to the transplanted gland was measured with a microsphere technique in anesthetized rats 2 weeks after transplantation. The animals were given an intravenous injection with saline alone, NG-nitro-L-arginine (25 mg/kg body weight) or sodium nitroprusside (10 micrograms/kg body weight) 10 min before blood flow measurements. Administration of NG-nitro-L-arginine increased mean arterial blood pressure and caused a pronounced decrease in whole pancreatic blood flow in both the native and transplanted gland. The islet blood flow was more markedly decreased by NG-nitro-L-arginine in both the native and transplanted pancreas, and constituted about 4% of whole pancreatic blood flow compared with 10% in the control animals. Sodium nitroprusside markedly decreased mean arterial blood pressure, but did not affect pancreatic or islet blood flow in any of the glands. It is concluded that inhibition of nitric oxide synthase causes a preferential decrease in islet blood flow both in the native pancreas and in the transplanted pancreas. This suggests that nitric oxide which affects islet blood flow is mainly endothelial-derived, and does not emanate from external nervous fibers.
Authors:
A M Svensson; S Sandler; L Jansson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  275     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-10     Completed Date:  1995-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-103     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Uppsala University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / antagonists & inhibitors*
Analysis of Variance
Animals
Arginine / administration & dosage,  analogs & derivatives*,  pharmacology
Blood Glucose / analysis,  drug effects
Blood Pressure / drug effects
Body Weight / drug effects
Duodenum / transplantation
Injections, Intravenous
Islets of Langerhans / blood supply*
Male
Microspheres
Nitric Oxide Synthase
Nitroarginine
Nitroprusside / administration & dosage,  pharmacology*
Organ Size / drug effects
Pancreas / blood supply,  drug effects,  metabolism
Pancreas Transplantation*
Rats
Rats, Inbred WF
Regional Blood Flow / drug effects
Chemical
Reg. No./Substance:
0/Blood Glucose; 15078-28-1/Nitroprusside; 2149-70-4/Nitroarginine; 74-79-3/Arginine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.4.-/Amino Acid Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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