Document Detail


A block in the road to fertility: autoantibodies to heat-shock protein 90-beta in human ovarian autoimmunity.
MedLine Citation:
PMID:  19022436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To report autoantibodies to human heat-shock protein 90-beta (HSP90 beta) in sera of women with infertility. DESIGN: Prospective, controlled observations. SETTING: Major urban infertility referral center and research institution. PATIENT(S): Fifty women with premature ovarian failure, 65 infertile women enrolled in the in vitro fertilization-embryo transfer program, and 60 normally menstruating fertile women as controls. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Identification and complete characterization of a 90-kd protein, the most immunodominant autoantigen. RESULT(S): Our previous studies employing a novel blocking demonstrated several cellular and molecular ovarian antigenic targets using patient's serum. Of all these antigens, the 90-kd protein designated as EP90 was found to be conserved across species, was serine-threonine phosphorylated, and was expressed from the primordial stage to the graafian-stage ooplasm of the oocytes during follicular development. Using high-throughput proteomic technologies like liquid chromatography/mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF), and tandem mass spectrometry analysis revealed the identity of this protein to be HSP90 beta. Commercially available recombinant protein immunoreacted with the sera from patients with antiovarian antibodies against the 90-kd antigen. In parallel, using monoclonal antibody to human HSP90, we found that it reacts with the eluted protein from a crude ovarian extract. CONCLUSION(S): This is the first report to show the presence of ovarian autoantibodies to human HSP90 in sera of women with infertility. This protein could be involved in human ovarian autoimmunity and thereby be a causative factor in early ovarian failure.
Authors:
Eusebio S Pires; Vrinda V Khole
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Publication Detail:
Type:  Journal Article     Date:  2008-11-19
Journal Detail:
Title:  Fertility and sterility     Volume:  92     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2009-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1395-409     Citation Subset:  IM    
Affiliation:
Department of Gamete Immuno Biology, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Parel, Mumbai, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantibodies / blood*,  isolation & purification,  metabolism
Autoimmune Diseases / complications,  immunology
Autoimmunity / immunology,  physiology
Case-Control Studies
Female
Fertility / immunology*
HSP90 Heat-Shock Proteins / immunology*
Humans
Infertility, Female / etiology*,  immunology
Mice
Ovarian Failure, Premature / blood,  complications,  immunology*
Ovary / immunology,  metabolism
Rabbits
Rats
Rats, Sprague-Dawley
Swine
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/HSP90 Heat-Shock Proteins; 0/HSP90AB1 protein, human

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