Document Detail

A block in release of progeny virus and a high particle-to-infectious unit ratio contribute to poor growth of enteric adenovirus types 40 and 41 in cell culture.
MedLine Citation:
PMID:  1373207     Owner:  NLM     Status:  MEDLINE    
The fastidious enteric adenovirus (FEAd) types 40 (Ad40) and 41 (Ad41) are found in stool specimens of infants and young children in association with gastroenteritis. Although they can be isolated routinely from clinical specimens by using 293 cells, they are propagated with variable success in cell lines which support the replication of other adenovirus serotypes. HeLa cells are generally considered to be nonpermissive for the replication of FEAds, but in this study, Ad40 and Ad41 grew to comparable titers in individual 293 and HeLa cells. However, virus was not efficiently released from infected HeLa cells and thus did not undergo multiple cycles of infection in HeLa cell cultures. The block in virus release was not overcome in KB18 cells which, like 293 cells, constitutively express proteins encoded by the E1B region of a subgroup C adenovirus (in this case Ad2). Moreover, it was apparent from these studies that Ad40 and Ad41 have particle-to-infectious unit ratios several orders of magnitude greater than that for Ad5, even in 293 cells which express the E1A and E1B proteins of Ad5 and are considered to be permissive for replication of the FEAds. Neither the block in release of progeny virus nor the high particle-to-infectious unit ratio is explained solely by the defect in expression of the E1B 55K protein identified by Mautner et al. (V. Mautner, N. MacKay, and V. Steinthorsdottir, Virology 171:619-622, 1989; V. Mautner, N. MacKay, and K. Morris, Virology 179:129-138, 1990).
M Brown; H L Wilson-Friesen; F Doane
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-14     Completed Date:  1992-05-14     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3198-205     Citation Subset:  IM    
Department of Microbiology, University of Toronto, Ontario, Canada.
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MeSH Terms
Adenovirus Early Proteins
Adenovirus Infections, Human / microbiology*
Adenoviruses, Human / growth & development*
Gastroenteritis / microbiology
Hela Cells
Interferons / pharmacology
Oncogene Proteins, Viral / biosynthesis
Virus Replication* / drug effects
Reg. No./Substance:
0/Adenovirus Early Proteins; 0/Oncogene Proteins, Viral; 9008-11-1/Interferons

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