| bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade. | |
MedLine Citation:
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PMID: 9006013 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The production of the red-pigmented tripyrrole antibiotic undecylprodigiosin (Red) by Streptomyces coelicolor A3(2) depends on two pathway-specific regulatory genes, redD and redZ. RedD is homologous to several other proteins that regulate antibiotic production in streptomycetes; RedZ is a member of the response regulator family. redZ transcripts were detected during exponential growth and increased in amount during transition and stationary phases; transcription of redD was confined to the two latter stages of growth. Whereas mutation of redD had no effect on redZ transcription, transcription of redD was highly dependent on redZ, suggesting that RedZ is a transcriptional activator of redD. bldA, which encodes the only tRNA of S. coelicolor that can efficiently translate the rare leucine codon UUA, is required for Red production at higher phosphate concentrations. While the redD transcript contains no UUA codons, the redZ mRNA contains one. Transcription of redZ appeared to be unaffected in a bldA mutant; in contrast, redD transcription was undetectable, consistent with the translational dependence of redZ on bldA and the transcriptional dependence of redD on redZ. Red production in a bldA mutant was restored by multiple copies of redZ, presumably reflecting a low level of mistranslation of the redZ UUA codon, while multiple copies of redD had no effect, presumably a consequence of the severe dependence of redD transcription on RedZ. Transcription of redZ appears to be negatively autoregulated. |
Authors:
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J White; M Bibb |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 179 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-02-28 Completed Date: 1997-02-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 627-33 Citation Subset: IM |
Affiliation:
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Department of Genetics, John Innes Centre, Norwich Research Park, Colney, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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biosynthesis* Bacterial Proteins* Codon Gene Expression Regulation, Bacterial* Genes, Bacterial Genes, Regulator Models, Genetic Mutagenesis Prodigiosin / analogs & derivatives, biosynthesis RNA, Bacterial RNA, Transfer, Leu / genetics* Streptomyces / genetics*, growth & development, metabolism Trans-Activators / biosynthesis* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/BldA tRNA, Streptomyces coelicolor; 0/Codon; 0/RNA, Bacterial; 0/RNA, Transfer, Leu; 0/RedZ protein, Streptomyces coelicolor; 0/Trans-Activators; 0/redD protein, Streptomyces coelicolor; 52340-48-4/undecylprodigiosin; 82-89-3/Prodigiosin |
| Comments/Corrections | |
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