Document Detail


bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade.
MedLine Citation:
PMID:  9006013     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The production of the red-pigmented tripyrrole antibiotic undecylprodigiosin (Red) by Streptomyces coelicolor A3(2) depends on two pathway-specific regulatory genes, redD and redZ. RedD is homologous to several other proteins that regulate antibiotic production in streptomycetes; RedZ is a member of the response regulator family. redZ transcripts were detected during exponential growth and increased in amount during transition and stationary phases; transcription of redD was confined to the two latter stages of growth. Whereas mutation of redD had no effect on redZ transcription, transcription of redD was highly dependent on redZ, suggesting that RedZ is a transcriptional activator of redD. bldA, which encodes the only tRNA of S. coelicolor that can efficiently translate the rare leucine codon UUA, is required for Red production at higher phosphate concentrations. While the redD transcript contains no UUA codons, the redZ mRNA contains one. Transcription of redZ appeared to be unaffected in a bldA mutant; in contrast, redD transcription was undetectable, consistent with the translational dependence of redZ on bldA and the transcriptional dependence of redD on redZ. Red production in a bldA mutant was restored by multiple copies of redZ, presumably reflecting a low level of mistranslation of the redZ UUA codon, while multiple copies of redD had no effect, presumably a consequence of the severe dependence of redD transcription on RedZ. Transcription of redZ appears to be negatively autoregulated.
Authors:
J White; M Bibb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  179     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-02-28     Completed Date:  1997-02-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  627-33     Citation Subset:  IM    
Affiliation:
Department of Genetics, John Innes Centre, Norwich Research Park, Colney, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / biosynthesis*
Bacterial Proteins*
Codon
Gene Expression Regulation, Bacterial*
Genes, Bacterial
Genes, Regulator
Models, Genetic
Mutagenesis
Prodigiosin / analogs & derivatives,  biosynthesis
RNA, Bacterial
RNA, Transfer, Leu / genetics*
Streptomyces / genetics*,  growth & development,  metabolism
Trans-Activators / biosynthesis*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/BldA tRNA, Streptomyces coelicolor; 0/Codon; 0/RNA, Bacterial; 0/RNA, Transfer, Leu; 0/RedZ protein, Streptomyces coelicolor; 0/Trans-Activators; 0/redD protein, Streptomyces coelicolor; 52340-48-4/undecylprodigiosin; 82-89-3/Prodigiosin
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