Document Detail


A biosynthetic method for the preparation of high specific activity 32P-labeled phospholipids.
MedLine Citation:
PMID:  993672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl-3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used.
Authors:
W D Marggraf
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  17     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-01-25     Completed Date:  1977-01-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  622-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Ehrlich Tumor / metabolism
Cattle
Cell Line
Cell Transformation, Neoplastic
Chromatography, Thin Layer
Cytidine Diphosphate Choline / biosynthesis
Erythrocytes / metabolism
Fibroblasts / metabolism
Humans
Isotope Labeling / methods
Leukocytes / metabolism
Mice
Phosphatidic Acids / biosynthesis
Phosphatidylcholines / biosynthesis
Phospholipids / biosynthesis*
Phosphorus Radioisotopes
Structure-Activity Relationship
Tritium
Chemical
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phospholipids; 0/Phosphorus Radioisotopes; 10028-17-8/Tritium; 987-78-0/Cytidine Diphosphate Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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