| A biosynthetic method for the preparation of high specific activity 32P-labeled phospholipids. | |
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MedLine Citation:
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PMID: 993672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl-3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used. |
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Authors:
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W D Marggraf |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of lipid research Volume: 17 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1976 Nov |
Date Detail:
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Created Date: 1977-01-25 Completed Date: 1977-01-25 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 622-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinoma, Ehrlich Tumor / metabolism Cattle Cell Line Cell Transformation, Neoplastic Chromatography, Thin Layer Cytidine Diphosphate Choline / biosynthesis Erythrocytes / metabolism Fibroblasts / metabolism Humans Isotope Labeling / methods Leukocytes / metabolism Mice Phosphatidic Acids / biosynthesis Phosphatidylcholines / biosynthesis Phospholipids / biosynthesis* Phosphorus Radioisotopes Structure-Activity Relationship Tritium |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phospholipids; 0/Phosphorus Radioisotopes; 10028-17-8/Tritium; 987-78-0/Cytidine Diphosphate Choline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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