Document Detail

The biosynthetic gene cluster for the 26-membered ring polyene macrolide pimaricin. A new polyketide synthase organization encoded by two subclusters separated by functionalization genes.
MedLine Citation:
PMID:  10187796     Owner:  NLM     Status:  MEDLINE    
The biosynthetic gene cluster for the 26-membered ring of the polyene macrolide pimaricin extends for about 110 kilobase pairs of contiguous DNA in the genome of Streptomyces natalensis. Two sets of polyketide synthase (PKS) genes are separated by a group of small polyketide-functionalizing genes. Two of the polyketide synthase genes, pimS0 and pimS1, have been fully sequenced and disrupted proving the involvement of each of these genes in pimaricin biosynthesis. The pimS0 gene encodes a relatively small acetate-activating PKS (approximately 193 kDa) that appears to work as a loading protein which "presents" the starter unit to the second PKS subunit. The pimS1 gene encodes a giant multienzyme (approximately 710 kDa) harboring 15 activities responsible for the first four cycles of chain elongation in pimaricin biosynthesis, resulting in formation of the polyene chromophore.
J F Aparicio; A J Colina; E Ceballos; J F Martín
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10133-9     Citation Subset:  IM    
Institute of Biotechnology INBIOTEC, Science Park of León, Avda. del Real, n degrees 1, 24006 León, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ132221;  AJ132222
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MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents / biosynthesis*,  chemistry
Binding Sites
Cloning, Molecular
DNA, Fungal / chemistry,  metabolism
Molecular Sequence Data
Multienzyme Complexes / genetics*,  metabolism
Natamycin / biosynthesis*,  chemistry
Sequence Alignment
Streptomyces / enzymology,  genetics*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Fungal; 0/Multienzyme Complexes; 7681-93-8/Natamycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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