Document Detail

A biomimetic phospholipid/alkanethiolate bilayer immobilizing uricase and an electron mediator on an Au electrode for amperometric determination of uric acid.
MedLine Citation:
PMID:  10517148     Owner:  NLM     Status:  MEDLINE    
A biomimetic bilayer membrane immobilizing uricase (urate oxidase; EC (UOx) and a redox agent of 1-methoxy-5-methylphenazinium (MMP) was fabricated on an Au electrode substrate with use of the Au substrate coated with a self-assembled monolayer of n-octanethiolate (OT/Au) and L-alpha-phosphatidylcholine beta-oleoyl-gamma-palmitoyl (PCOP). The preparation was carried out by successively immersing an Au electrode substrate in an ethanol solution of OT, an MMP aqueous solution, and a suspension of proteoliposome formed by PCOP containing UOx and MMP. The prepared electrode exhibited such fast steady amperometric responses to uric acid as to allow its determination within 20 s after injecting uric acid, indicating that UOx-catalyzed electrochemical oxidation of uric acid was accomplished with assistance of electron mediation by MMP between UOx and the Au substrate. An increase in the response currents with increasing concentration of uric acid was obtained in a concentration range of uric acid found in healthy human blood. Any interference in the current response that is caused by direct anodic oxidation of uric acid or ascorbic acid was not observed at the prepared sensor electrode because the densely packed bilayer effectively blocked the diffusion of these substrates toward the Au surface, making it possible to determine amperometrically uric acid at the electrode with high precision.
T Nakaminami; S Ito; S Kuwabata; H Yoneyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  2000-02-01     Completed Date:  2000-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4278-83     Citation Subset:  IM    
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Japan.
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MeSH Terms
Electrochemistry / instrumentation,  methods*
Enzymes, Immobilized / chemistry*,  metabolism
Lipid Bilayers
Methylphenazonium Methosulfate / analogs & derivatives,  chemistry
Phosphatidylcholines / chemistry
Phospholipids / chemistry
Reference Values
Sensitivity and Specificity
Sulfhydryl Compounds / chemistry
Urate Oxidase / chemistry*,  metabolism
Uric Acid / analysis*,  blood
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Lipid Bilayers; 0/Phosphatidylcholines; 0/Phospholipids; 0/Sulfhydryl Compounds; 111-88-6/n-octanethiol; 299-11-6/Methylphenazonium Methosulfate; 65162-13-2/1-methoxy-5-methylphenazinium methyl sulfate; 6753-55-5/1-palmitoyl-2-oleoylphosphatidylcholine; 69-93-2/Uric Acid; 7440-57-5/Gold; EC Oxidase

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