Document Detail

A bioluminescent HL-60 cell line to assay anti-leukaemia therapeutics under physiological conditions.
MedLine Citation:
PMID:  18167057     Owner:  NLM     Status:  MEDLINE    
Screens for compounds and proteins with anti-cancer activity employ viability assays using relevant cancer cell lines. For leukaemia studies, the human leukaemia cell line, HL-60, is often used as a model system. To facilitate the discovery and investigation of anti-leukaemia therapeutics under physiological conditions, we have engineered HL-60 cells that stably express firefly luciferase and produce light that can be detected using an in vivo imaging system (IVIS). Bioluminescent HL-60luc cells could be rapidly detected in whole blood with a sensitivity of approximately 1000 viable cells/200 microl blood. Treatment of HL-60luc cells with the drug chlorambucil revealed that the bioluminescent viability assay is able to detect cell death earlier than the Trypan blue dye exclusion assay. HL-60luc cells administered intraperitoneally (i.p.) or intravenously (i.v.) were visualized in living mice. The rapidity and ease of detecting HL-60luc cells in biological fluid indicates that this cell line could be used in high-throughput screens for the identification of drugs with anti-leukaemia activity under physiological conditions.
Maria P Isaza; Jennie T Chau; Amy Le; Nataliya V Balashova; Jigna K Patel; Erica Salerno; Juan A Crosby; Anna O'Connor; Scott C Kachlany
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  23     ISSN:  1522-7243     ISO Abbreviation:  Luminescence     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-04-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  England    
Other Details:
Languages:  eng     Pagination:  17-21     Citation Subset:  IM    
Copyright Information:
(c) 2007 John Wiley & Sons, Ltd.
Department of Oral Biology, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA.
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MeSH Terms
Antineoplastic Agents / chemistry*,  therapeutic use
Cell Survival / drug effects
Disease Models, Animal
Firefly Luciferin / administration & dosage,  chemistry*
Gene Expression Regulation, Enzymologic
HL-60 Cells
Leukemia / drug therapy*
Luciferases, Firefly / chemistry,  genetics
Luminescent Measurements
Mice, SCID
Neoplasm Transplantation
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents; 2591-17-5/Firefly Luciferin; EC, Firefly

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