Document Detail


A bioassay for the measurement of insecticide concentration.
MedLine Citation:
PMID:  11503068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bioassay was developed to measure insecticide residues using fruit flies (Drosophila melongaster). After adding a known volume of sampling solution, the time at which 50% of the flies were dead (LT(50)) was recorded and cross-referenced to the appropriate calibration curve. Using known standards, comparable results were obtained using the bioassay and GC-MS. The bioassay allows concentrations of synthetic pyrethroids as low as 1 pg L(-1) to be measured with a variance of < 5%. The bioassay can be used reliably over a wide range of temperatures and it is tolerant to a range of pH and surface tensions of the test solution. The whole bioassay is compact, physically robust, and simple to use; hence, it could be of use in the field as a quick preliminary assessment of water contamination.
Authors:
R J Grant
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Archives of environmental contamination and toxicology     Volume:  41     ISSN:  0090-4341     ISO Abbreviation:  Arch. Environ. Contam. Toxicol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-08-14     Completed Date:  2001-10-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0357245     Medline TA:  Arch Environ Contam Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Affiliation:
Department of Biology, University of York, Heslington, York YO10 5DD, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods
Calibration
Drosophila melanogaster / drug effects
Environmental Monitoring / methods*
Hydrogen-Ion Concentration
Insecticides / analysis*,  toxicity*
Lethal Dose 50
Surface Tension
Chemical
Reg. No./Substance:
0/Insecticides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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