| CCAAT/enhancer-binding protein-delta (C/EBP-delta) is induced in growth-arrested mouse mammary epithelial cells. | |
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MedLine Citation:
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PMID: 9045647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CCAAT/enhancer binding proteins (C/EBPs) are a highly conserved family of DNA-binding proteins that regulate cell growth and differentiation in a highly tissue-specific manner. These experiments investigated the influence of the cell cycle on C/EBP isoform expression in mammary epithelial cells (COMMA D) and fibroblasts (NIH3T3). C/EBP-delta gene expression is induced in COMMA D cells arrested in G0 by serum and growth factor withdrawal or contact inhibition. C/EBP-delta mRNA, nuclear protein content, and DNA binding activity increase during G0 growth arrest and decrease after cell cycle induction in COMMA D cells. Growth arrest is markedly delayed in COMMA D cells expressing a C/EBP-delta antisense construct. C/EBP-beta is induced during G1 of the cell cycle. In contrast to COMMA D cells, C/EBP-beta and C/EBP-delta mRNA levels remain relatively constant in growth-arrested and cell cycle-induced NIH3T3 cells. However, C/EBP homologous protein (CHOP10) mRNA levels markedly increase in growth-arrested NIH3T3 cells. Both COMMA D and NIH3T3 cells express growth arrest-specific (gas1) and JunD during G0. These results demonstrate that COMMA D and NIH3T3 cells achieve a common growth arrest (G0) state by cell-specific strategies that involve the induction of different C/EBP isoforms. |
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Authors:
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J O'Rourke; R Yuan; J DeWille |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 272 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1997 Mar |
Date Detail:
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Created Date: 1997-04-14 Completed Date: 1997-04-14 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6291-6 Citation Subset: IM |
Affiliation:
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Ohio State University Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals CCAAT-Enhancer-Binding Protein-delta CCAAT-Enhancer-Binding Proteins* Cell Compartmentation Cell Cycle Proteins Cell Division* Cell Nucleus / metabolism Cells, Cultured Cytoplasm / metabolism DNA-Binding Proteins / metabolism* Female Gene Expression Regulation, Developmental Histones / metabolism Mammary Glands, Animal / cytology* Membrane Proteins / metabolism Mice Nuclear Proteins / metabolism* Peptides / chemistry, immunology RNA, Messenger / genetics Transcription Factors / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/CCAAT-Enhancer-Binding Proteins; 0/Cebpd protein, mouse; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/GAS1 protein, human; 0/Gas1 protein, mouse; 0/Histones; 0/Membrane Proteins; 0/Nuclear Proteins; 0/Peptides; 0/RNA, Messenger; 0/Transcription Factors; 142662-43-9/CCAAT-Enhancer-Binding Protein-delta |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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