Document Detail


CCAAT/enhancer-binding protein-delta (C/EBP-delta) is induced in growth-arrested mouse mammary epithelial cells.
MedLine Citation:
PMID:  9045647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CCAAT/enhancer binding proteins (C/EBPs) are a highly conserved family of DNA-binding proteins that regulate cell growth and differentiation in a highly tissue-specific manner. These experiments investigated the influence of the cell cycle on C/EBP isoform expression in mammary epithelial cells (COMMA D) and fibroblasts (NIH3T3). C/EBP-delta gene expression is induced in COMMA D cells arrested in G0 by serum and growth factor withdrawal or contact inhibition. C/EBP-delta mRNA, nuclear protein content, and DNA binding activity increase during G0 growth arrest and decrease after cell cycle induction in COMMA D cells. Growth arrest is markedly delayed in COMMA D cells expressing a C/EBP-delta antisense construct. C/EBP-beta is induced during G1 of the cell cycle. In contrast to COMMA D cells, C/EBP-beta and C/EBP-delta mRNA levels remain relatively constant in growth-arrested and cell cycle-induced NIH3T3 cells. However, C/EBP homologous protein (CHOP10) mRNA levels markedly increase in growth-arrested NIH3T3 cells. Both COMMA D and NIH3T3 cells express growth arrest-specific (gas1) and JunD during G0. These results demonstrate that COMMA D and NIH3T3 cells achieve a common growth arrest (G0) state by cell-specific strategies that involve the induction of different C/EBP isoforms.
Authors:
J O'Rourke; R Yuan; J DeWille
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-04-14     Completed Date:  1997-04-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6291-6     Citation Subset:  IM    
Affiliation:
Ohio State University Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
CCAAT-Enhancer-Binding Protein-delta
CCAAT-Enhancer-Binding Proteins*
Cell Compartmentation
Cell Cycle Proteins
Cell Division*
Cell Nucleus / metabolism
Cells, Cultured
Cytoplasm / metabolism
DNA-Binding Proteins / metabolism*
Female
Gene Expression Regulation, Developmental
Histones / metabolism
Mammary Glands, Animal / cytology*
Membrane Proteins / metabolism
Mice
Nuclear Proteins / metabolism*
Peptides / chemistry,  immunology
RNA, Messenger / genetics
Transcription Factors / metabolism*
Chemical
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/Cebpd protein, mouse; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/GAS1 protein, human; 0/Gas1 protein, mouse; 0/Histones; 0/Membrane Proteins; 0/Nuclear Proteins; 0/Peptides; 0/RNA, Messenger; 0/Transcription Factors; 142662-43-9/CCAAT-Enhancer-Binding Protein-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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