Document Detail


The binding protein BiP attenuates stress-induced cell death in soybean via modulation of the N-rich protein-mediated signaling pathway.
MedLine Citation:
PMID:  22007022     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The molecular chaperone binding protein (BiP) participates in the constitutive function of the endoplasmic reticulum (ER)and protects the cell against stresses. In this study, we investigated the underlying mechanism by which BiP protects plant cells from stress-induced cell death. We found that enhanced expression of BiP in soybean (Glycine max) attenuated ER-stress- and osmotic-stress-mediated cell death. Ectopic expression of BiP in transgenic lines attenuated the leaf necrotic lesions that are caused by the ER stress inducer tunicamycin and also maintained shoot turgidity upon polyethylene glycol (PEG)-induced dehydration. BiP-mediated attenuation of stress-induced cell death was confirmed by the decreased percentage of dead cell, the reduced induction of the senescence-associated marker gene GmCystP and reduced DNA fragmentation in BiP-overexpressing lines. These phenotypes were accompanied by a delay in the induction of the cell death marker genes NRP-A, NRP-B and GmNAC6, which are involved in transducing a cell death signal generated by ER stress and osmotic stress through the N-rich protein (NRP)-mediated signaling pathway. The prosurvival effect of BiP was associated with modulation of the ER-stress- and osmotic-stress-induced NRP-mediated cell death signaling, as determined in transgenic tobacco lines with enhanced (sense) and suppressed (antisense) BiP levels. Enhanced expression of BiP prevented NRP- and NAC6-mediated chlorosis and the appearance of senescence-associated markers, whereas silencing of endogenous BiP accelerated the onset of leaf senescence mediated by NRPs and GmNAC6. Collectively, these results implicate BiP as a negative regulator of the stress-induced NRP-mediated cell death response.
Authors:
Pedro A B Reis; Gustavo L Rosado; Lucas A C Silva; Luciana C Oliveira; Lucas B Oliveira; Maximiller D L Costa; Fatima C Alvim; Elizabeth P B Fontes
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-17
Journal Detail:
Title:  Plant physiology     Volume:  -     ISSN:  1532-2548     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Universidade Federal de Vicosa;
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