| A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells. | |
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MedLine Citation:
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PMID: 18541133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells. |
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Authors:
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Andreas Lackner; Kathrin Genta; Herwig Koppensteiner; Irene Herbacek; Klaus Holzmann; Sabine Spiegl-Kreinecker; Walter Berger; Michael Grusch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-22 |
Journal Detail:
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Title: Analytical biochemistry Volume: 380 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-07-14 Completed Date: 2008-10-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 146-8 Citation Subset: IM |
Affiliation:
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Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae / genetics* Cell Line, Tumor Gene Expression Regulation* Gene Transfer Techniques Genetic Vectors / genetics* Glioblastoma / genetics, metabolism, pathology Humans Proteins / genetics* Time Factors Transduction, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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