Document Detail


A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells.
MedLine Citation:
PMID:  18541133     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.
Authors:
Andreas Lackner; Kathrin Genta; Herwig Koppensteiner; Irene Herbacek; Klaus Holzmann; Sabine Spiegl-Kreinecker; Walter Berger; Michael Grusch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-22
Journal Detail:
Title:  Analytical biochemistry     Volume:  380     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  146-8     Citation Subset:  IM    
Affiliation:
Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Baculoviridae / genetics*
Cell Line, Tumor
Gene Expression Regulation*
Gene Transfer Techniques
Genetic Vectors / genetics*
Glioblastoma / genetics,  metabolism,  pathology
Humans
Proteins / genetics*
Time Factors
Transduction, Genetic
Chemical
Reg. No./Substance:
0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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