Document Detail


Beta1,4-galactosyltransferase V regulates self-renewal of glioma-initiating cell.
MedLine Citation:
PMID:  20417617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glioma results from unregulated expansion of a self-renewing glioma-initiating cell population. The regulatory pathways which are essential for sustaining the self-renewal of glioma-initiating cells remain largely unknown. Cell surface N-linked oligosaccharides play functional roles in determining cell fate and are associated with glioma malignancy. Previously, we have reported that beta1,4-galactosyltransferase V (beta1,4GalT V) effectively galactosylates the GlcNAcbeta1-->6Man arm of the highly branched N-glycans and positively regulates glioma cell growth. Here, we show that decreasing the expression of beta1,4GalT V by RNA interference in glioma cells attenuated the formation of polylactosamine and inhibited the ability of tumor formation in vivo. Down-regulation of beta1,4GalT V depleted CD133-positive cells in glioma xenograft, and inhibited the self-renewal capacity and the tumorigenic potential of glioma-initiating cells. These data reveal a critical role of beta1,4GalT V in the self-renewal and tumorigenicity of glioma-initiating cells, and indicate that manipulating beta1,4GalT V expression may have therapeutic potential for the treatment of malignant glioma.
Authors:
Yuanyan Wei; Fengbiao Zhou; Yuqing Ge; Hong Chen; Chunhong Cui; Qiuping Li; Dan Liu; Zhiyuan Yang; Guoqiang Wu; Shuhui Sun; Jianxin Gu; Jianhai Jiang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-24
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  396     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-07-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  602-7     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Affiliation:
Key Laboratory of Glycoconjuates Research, Ministry of Public Health & Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Amino Sugars / metabolism
Animals
Cell Line, Tumor
Galactosyltransferases / genetics,  physiology*
Gene Knockdown Techniques
Glioma / enzymology,  pathology*
Humans
Mice
Mice, Nude
Neoplastic Stem Cells / enzymology,  pathology*
Polysaccharides / metabolism
RNA, Small Interfering / genetics
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Amino Sugars; 0/Polysaccharides; 0/RNA, Small Interfering; 100787-31-3/polylactosamine; EC 2.4.1.-/Galactosyltransferases; EC 2.4.1.-/beta-1,4-galactosyltransferase V

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