Document Detail


beta-xylosidase activity and expression of a beta-xylosidase gene during strawberry fruit ripening.
MedLine Citation:
PMID:  15283123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of beta-xylosidase and the expression of a beta-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative beta-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other beta-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25-50% red fruit and decreasing thereafter. The total beta-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25-50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other beta-xylosidases-like genes. Both the expression of FcaXyl1 and the total beta-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and beta-xylosidase is discussed.
Authors:
Gustavo A Martínez; Alicia R Chaves; Pedro M Civello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  42     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-07-30     Completed Date:  2004-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  89-96     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH) (CONICET-UNSAM), Camino de Circunvalación Laguna Km 6. 7130 Chascomús, Argentina. gmartinez@intech.gov.ar
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cellulases / genetics
Cloning, Molecular
Conserved Sequence
DNA, Complementary / genetics
Fragaria / enzymology,  genetics,  growth & development*
Gene Expression Regulation, Plant / genetics
Molecular Sequence Data
Phylogeny
Sequence Alignment
Sequence Homology, Amino Acid
Xylosidases / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/DNA, Complementary; EC 3.2.1.-/Cellulases; EC 3.2.1.-/Xylosidases; EC 3.2.1.37/exo-1,4-beta-D-xylosidase

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