| beta-lactoglobulin under high pressure studied by small-angle neutron scattering. | |
| | |
MedLine Citation:
|
PMID: 16337234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We used small-angle neutron scattering to study the effects of the high hydrostatic pressure on the structure of beta-lactoglobulin. Experiments were carried out at pH 7 on the dimeric form of the protein in a pressure range going from 50 MPa to 300 MPa. These measurements allow the protein size and the interactions between macromolecules to be studied during the application of pressure. Increasing pressure up to 150 MPa leads to a swollen state of the protein that gives rise to an increase of the radius of gyration by about 7%. Within this pressure range, we also show that the interaction between macromolecules weakens although it remains repulsive. The measurements show an aggregation process occurring above 150 MPa. From the spectra analysis, it appears that the aggregation occurs mainly by association of the dimeric units. |
| | |
Authors:
|
C Loupiac; M Bonetti; S Pin; P Calmettes |
Related Documents
:
|
19937284 - Reduced pressure ice fog technique for controlled ice nucleation during freeze-drying. 16509654 - Thermal decomposition of cloocl. 14672224 - Sensitivity of vibrio species in phosphate-buffered saline and in oysters to high-press... 20461224 - Rapid inactivation of penicillium digitatum spores using high-density nonequilibrium at... 2937724 - Cardiovascular effects of atrial natriuretic factor in anesthetized and conscious dogs. 18247974 - Correlation between primary and secondary johari-goldstein relaxations in supercooled l... |
Publication Detail:
|
Type: Journal Article Date: 2005-11-07 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1764 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 Feb |
Date Detail:
|
Created Date: 2006-02-28 Completed Date: 2006-04-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 211-6 Citation Subset: IM |
Affiliation:
|
Equipe d'Ingénierie Moléculaire et Sensorielle des Aliments et des Produits de Santé, ENSBANA, Dijon, France. camille.loupiac@u-bourgogne.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Dimerization Hydrogen-Ion Concentration Hydrostatic Pressure Lactoglobulins / chemistry* Neutron Diffraction Protein Folding Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
|
0/Lactoglobulins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Probing the pressure-temperature stability of amyloid fibrils provides new insights into their molec...
Next Document: Dissociative disorders among adults in the community, impaired functioning, and axis I and II comorb...