Document Detail


beta-catenin expression and mutational analysis in renal cell carcinomas.
MedLine Citation:
PMID:  11012986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
beta-Catenin acts as a downstream transcriptional activator of the Wingless-Wnt signaling pathway. The beta-catenin-Tcf complex transactivates the downstream genes that regulate cell proliferation or inhibit apoptosis. The activation of this pathway through stabilization of beta-catenin is caused either by inactivating mutations of adenomatous polyposis coli (APC) tumor suppressor gene or by activating mutations in beta-catenin exon 3. To determine whether the abnormal expression and activating mutations in exon 3 of the beta-catenin gene are implicated in renal cell carcinogenesis, 52 renal cell carcinomas (RCC) were analyzed by immunohistochemistry, polymerase chain reaction-single-strand conformational polymorphism analysis (PCR-SSCP), and direct DNA sequencing. Immunohistochemically, all cases, as well as normal kidneys, showed membranous and/or cytoplasmic staining patterns without nuclear localization. However, the cytoplasmic accumulations of beta-catenin were observed in five (22.7%) of 22 cases of conventional (clear cell) renal carcinoma, but not in papillary or chromophobe renal carcinomas. The beta-catenin mutation was identified in only one case of conventional renal carcinoma and was a single-base missense mutation on codon 61, leading to substitution of glutamine by arginine. In conclusion, this study demonstrates that beta-catenin mutations are a relatively rare event in RCC and that cytoplasmic accumulations of beta-catenin protein are found only in conventional (clear cell) renal carcinomas. These data suggest that the activation of the beta-catenin signaling pathway may partly play a role in the development of conventional RCC.
Authors:
Y S Kim; Y K Kang; J B Kim; S A Han; K I Kim; S R Paik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology international     Volume:  50     ISSN:  1320-5463     ISO Abbreviation:  Pathol. Int.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-06     Completed Date:  2000-11-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  725-30     Citation Subset:  IM    
Affiliation:
Department of Pathology, College of Medicine, Korea University, Gojan-Dong, Ansan, Korea. apysk@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics,  metabolism,  pathology
Adult
Aged
Carcinoma, Papillary / genetics,  metabolism,  pathology
Carcinoma, Renal Cell / genetics*,  metabolism,  pathology
Cytoskeletal Proteins / biosynthesis,  genetics*
DNA Mutational Analysis
DNA Primers / chemistry
DNA, Neoplasm* / analysis
Female
Humans
Immunoenzyme Techniques
Kidney / metabolism,  pathology
Kidney Neoplasms / genetics*,  metabolism,  pathology
Male
Middle Aged
Mutation, Missense
Neoplasm Staging
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Trans-Activators*
beta Catenin
Chemical
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cytoskeletal Proteins; 0/DNA Primers; 0/DNA, Neoplasm; 0/Trans-Activators; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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