Document Detail

beta-Xyloside effects on basal lamina structure and anionic site distribution the embryonic mouse submandibular salivary gland.
MedLine Citation:
PMID:  2480774     Owner:  NLM     Status:  MEDLINE    
beta-D-Xyloside is a proteoglycan biosynthesis inhibitor. Previous studies on embryonic salivary glands have demonstrated that 0.5 mM beta-xyloside (1) inhibits proteoglycan synthesis by 50%; (2) severely depresses sulphated glycosaminoglycan deposition at the basal epithelial surface, and (3) dramatically inhibits epithelial branching morphogenesis. Electron microscopy revealed a conventional three-layered basal lamina that is altered in the presence of beta-xyloside by a 35% reduction in the number of tannic acid-resolved particles in the lamina densa. Basal lamina anionic sites, resolved with ruthenium red (RR) and polyethyleneimine (PEI) cationic probes, were also reduced in the presence of beta-xyloside. PEI particles were reduced by 28%, and RR particles by 24%, per two-dimensional unit of basal lamina. These beta-xyloside effects on anionic sites are consistent with an hypothesis that sulphated glycosaminoglycans account for 50% of the basal lamina anionic sites and a predicted 25% decrease in anionic sites in the presence of beta-xyloside.
B S Spooner; A Paulsen; M S Sullins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of oral biology     Volume:  34     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1989  
Date Detail:
Created Date:  1990-01-17     Completed Date:  1990-01-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  541-9     Citation Subset:  D; IM    
Division of Biology, Kansas State University, Manhattan 66506.
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MeSH Terms
Basement Membrane / drug effects,  embryology*,  ultrastructure
Cells, Cultured
Glycosaminoglycans / biosynthesis
Glycosides / pharmacology*
Hydrolyzable Tannins
Ion Channels / drug effects*
Microscopy, Electron
Ruthenium Red
Submandibular Gland / drug effects,  embryology*,  ultrastructure
Grant Support
Reg. No./Substance:
0/Glycosaminoglycans; 0/Glycosides; 0/Hydrolyzable Tannins; 0/Ion Channels; 0/xylosides; 11103-72-3/Ruthenium Red; 9002-98-6/Polyethyleneimine

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