| β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. | |
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MedLine Citation:
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PMID: 20731356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays a central role in the pathogenesis of atherosclerosis. β-Sitosterol, an important phytosterol found in plant food, is known to exert antiatherosclerosis activity. However, the molecular mechanisms underlying β-sitosterol-induced antiproliferation of VSMCs were still not clear. This study demonstrated that β-sitosterol (1-20 μM) concentration-dependently inhibited proliferation of rat aortic smooth muscle cells (RASMCs) without cytotoxic effect. Flow cytometric analysis revealed that β-sitosterol arrested cell cycle progression through down-regulation of cyclin E and cyclin-dependent kinase (CDK)2 and up-regulation of p21cip1. In the β-sitosterol-treated RASMCs, the formation of the CDK2-p21cip1 complex was increased and the assayable CDK2 activity was decreased. Knockdown of the expression of p21cip1 gene prevented β-sitosterol-induced cell cycle arrest in RASMCs. In conclusion, β-sitosterol inhibited VSMC proliferation by increasing the levels of p21cip1 protein, which in turn inhibited the CDK2 activity, and finally interrupted the progress of the cell cycle. |
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Authors:
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Ming-Hsien Chien; Tong-Sheng Lee; Yu-Chih Liang; Wen-Sen Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2011-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 10064-9 Citation Subset: IM |
Affiliation:
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Taipei Medical University-Wang-Fang Hospital, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / cytology, drug effects*, metabolism Atherosclerosis / prevention & control Cell Cycle / drug effects* Cell Proliferation / drug effects Cells, Cultured Cyclin-Dependent Kinase Inhibitor p21 / genetics, metabolism* Hypolipidemic Agents / adverse effects, pharmacology* Male Muscle, Smooth, Vascular / cytology, drug effects*, metabolism Rats Rats, Sprague-Dawley Sitosterols / adverse effects, pharmacology* Up-Regulation / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Cdkn1a protein, rat; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Hypolipidemic Agents; 0/Sitosterols; 5779-62-4/sitosterol |
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