| beta-N-acetylglucosaminidase in the reproductive organs and seminal plasma of the bull. | |
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MedLine Citation:
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PMID: 3944796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The highest specific activity of beta-N-acetylglucosaminidase (beta-NAG) was found in the different parts of the epididymis, where the activity seemed to be partly in secretory and partly in non-secretory, tissue-bound form. Epididymal spermatozoa also contained moderate beta-NAG activity. The beta-NAG was separated by chromatofocussing and anion exchange chromatography with HPLC into multiple forms with distinct pI values (8.0-4.0). The cauda epididymidis, ampulla and the seminal vesicles formed the major secretory sources of the high beta-NAG activity in bull seminal plasma. The major secretory forms of beta-NAG in caput and cauda epididymidis showed distinct elution profiles. In the fractionation with gel filtration on Sepharose 6B, the beta-NAG activities derived from bull testis and caput epididymidis had smaller molecular weights than did the secretory enzymes in seminal plasma, seminal vesicle secretion and cauda epididymidis. Maximum activity of all beta-NAG isoenzymes was observed at pH 5.0. They were almost totally inactivated at 60 degrees C and about 75-80% of the activity was lost at 55 degrees C. All the isoenzymes were strongly inhibited by thiol reagents but not with other metal ions and chelating agents. Histochemical studies showed a strong granular (lysosomal) reaction for beta-NAG in basal cells and basal parts of the principal cells in all but the initial segment of the epididymis. An apical (secretory) reaction was prominent in the distal caput and corpus as well as in distal cauda. After the distal caput the luminal sperm mass became increasingly mixed with a beta-NAG-positive material. The epithelial cells of the ampulla and seminal vesicle displayed a moderate apical (secretory) reaction. |
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Authors:
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A Jauhiainen; T Vanha-Perttula |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 76 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-03-07 Completed Date: 1986-03-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 239-50 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosaminidase
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analysis*,
secretion Animals Cattle Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Epididymis / enzymology*, secretion Hexosaminidases / analysis* Isoelectric Focusing Isoenzymes / analysis* Male Semen / enzymology* Seminal Vesicles / secretion Spermatozoa / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 3.2.1.-/Hexosaminidases; EC 3.2.1.52/Acetylglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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