| {beta}-Myosin Heavy Chain Is Induced by Pressure Overload in a Minor Subpopulation of Smaller Mouse Cardiac Myocytes. | |
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MedLine Citation:
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PMID: 21778428 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Rationale: Induction of the fetal hypertrophic marker gene β-myosin heavy chain (β-MyHC) is a signature feature of pressure overload hypertrophy in rodents. β-MyHC is assumed present in all or most enlarged myocytes. Objective: To quantify the number and size of myocytes expressing endogenous β-MyHC by a flow cytometry approach. Methods and Results: Myocytes were isolated from the left ventricle of male C57Bl/6J mice after transverse aortic constriction (TAC), and the fraction of cells expressing endogenous β-MyHC was quantified by flow cytometry on 10 000 to 20 000 myocytes with use of a validated β-MyHC antibody. Side scatter by flow cytometry in the same cells was validated as an index of myocyte size. β-MyHC(+) myocytes constituted 3±1% of myocytes in control hearts (n=12), increasing to 25±10% at 3 days to 6 weeks after TAC (n=24, P<0.01). β-MyHC(+) myocytes did not enlarge with TAC and were smaller at all times than myocytes without β-MyHC (≈70% as large, P<0.001). β-MyHC(+) myocytes arose by addition of β-MyHC to α-MyHC and had more total MyHC after TAC than did the hypertrophied myocytes that had α-MyHC only. Myocytes positive for β-MyHC were found in discrete regions of the left ventricle in 3 patterns: perivascular, in areas with fibrosis, and in apparently normal myocardium. Conclusions: β-MyHC protein is induced by pressure overload in a minor subpopulation of smaller cardiac myocytes. The hypertrophied myocytes after TAC have α-MyHC only. These data challenge the current paradigm of the fetal hypertrophic gene program and identify a new subpopulation of smaller working ventricular myocytes with more myosin. |
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Authors:
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Javier E López; Bat-Erdene Myagmar; Philip M Swigart; Megan D Montgomery; Stephen Haynam; Marty Bigos; Manoj C Rodrigo; Paul C Simpson |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-21 |
Journal Detail:
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Title: Circulation research Volume: - ISSN: 1524-4571 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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VA Medical Center. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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