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[beta]-Glucan Synthesis in the Cotton Fiber (II. Regulation and Kinetic Properties of [beta]-Glucan Synthases.
MedLine Citation:
PMID:  12231765     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The regulation and kinetic properties of cellulose synthase as well as [beta]-1,3-glucan synthase have been studied. The cellulose was detected using acetic/nitric acid insolubility as an indicator of cellulose (this product contained only [beta]-1,4-linked glucans; K. Okuda, L. Li, K. Kudlicka, S. Kuga, R.M. Brown, Jr. [1993] Plant Physiol 101: 1131-1142). These studies reveal that (a) [beta]-1,3-glucan synthesis is enhanced up to 31-fold by cellobiose with a Ka of 1.16 mM; (b) cellulose synthesis is increased 12-fold by a combination of cellobiose (Ka = 3.26 mM) and cyclic-3[prime]:5[prime]-GMP (Ka = 100 [mu]M); (c) the common components in the reaction mixture required by both enzymes are cellobiose, calcium, and digitonin; (d) cellulose synthase has an essential requirement for magnesium (Ka = 0.89 mM); (e) cellulose synthase also requires a low concentration of calcium (Ka = 90 [mu]M); (f) the optimal pH for cellulose synthase (7.6-8.0) is slightly higher than that for [beta]-1,3-glucan synthase (7.2-7.6); (g) the Km for UGP-Glc for cotton (Gossypium hirsutum) cellulose synthase is 0.40 mM; (h) the Km for UDP-Glc for for [beta]-1,3-glucan synthase is 0.43 mM.
Authors:
L. Li; R. M. Brown
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Plant physiology     Volume:  101     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  2002-Sep-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1143-1148     Citation Subset:  -    
Affiliation:
Department of Botany, The University of Texas at Austin, Austin, Texas 78713-7640.
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