| beta-Galactosidase transgene expression in transplanted rabbit retinal pigment epithelial cells in vivo. | |
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MedLine Citation:
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PMID: 7797086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Intraocular transplantation of genetically modified cells that release a particular substance could have a major impact on the treatment of various ocular diseases. We studied the expression of the reporter gene beta-galactosidase (lacZ) in transplanted retinal pigment epithelial (RPE) cells in vivo. METHODS: RPE cells from pigmented rabbits were transduced with the beta-galactosidase gene in a retroviral vector. Cells were then assayed for gene expression and transplanted subretinally into the eyes of New Zealand White rabbits. RPE cells that were transduced with a similar vector without the beta-galactosidase gene were used as controls. Rabbits were killed on days 1, 7, and 21 and the eyes processed for transmission electron microscopy RESULTS: Neomycin-resistant rabbit RPE cells that showed beta-galactosidase activity were generated within 2-5 weeks. After transplantation, viable RPE cells that expressed the transgene and that phagocytosed rod outer segments were observed on days 1, 7, and 21 CONCLUSIONS: The results show that generation of genetically modified RPE cells is feasible and that the transplanted cells remain viable and continue to express the transgene in the subretinal space of the host animal for at least 21 days. Transplantation of such genetically modified RPE cells could provide a new tool for studying retinal diseases and, potentially, for correcting metabolic abnormalities in retinal degenerations and dystrophies. |
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Authors:
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R Osusky; M Jiang; E R Büchi; C Spee; J Ye; S J Ryan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Volume: 233 ISSN: 0721-832X ISO Abbreviation: Graefes Arch. Clin. Exp. Ophthalmol. Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-08-02 Completed Date: 1995-08-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8205248 Medline TA: Graefes Arch Clin Exp Ophthalmol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 220-5 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, University of Southern California School of Medicine, Los Angeles, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Bruch Membrane / surgery Cells, Cultured Fundus Oculi Gene Expression Regulation, Enzymologic / physiology* Gene Transfer Techniques* Genetic Vectors Mice Pigment Epithelium of Eye / cytology, enzymology*, transplantation*, ultrastructure Plasmids Rabbits beta-Galactosidase / biosynthesis*, genetics* |
| Grant Support | |
ID/Acronym/Agency:
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EYO-1545/EY/NEI NIH HHS; EYO-3040/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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