Document Detail

beta-Conglycinins among sources of bioactives in hydrolysates of different soybean varieties that inhibit leukemia cells in vitro.
MedLine Citation:
PMID:  18473471     Owner:  NLM     Status:  MEDLINE    
Soybean is a complex matrix containing several potentially bioactive components. The objective was to develop a statistical model to predict the in vitro anticancer potential of soybean varieties based on the correlation between protein composition and bioactive components after simulated gastrointestinal enzyme digestion with their effect on leukemia mouse cells. The IC 50 values of the hydrolysates of soy genotypes (NB1-NB7) on L1210 leukemia cells ranged from 3.5 to 6.2 mg/mL. Depending on genotype, each gram of soy hydrolysates contained 2.7-6.6 micromol of total daidzein, 3.0-4.7 micromol of total genistein, 0.5-1.3 micromol of glycitein, 2.1-2.8 micromol of total saponins, 0.1-0.2 micromol of lunasin, and 0.1-0.6 micromol of Bowman-Birk inhibitor (BBI). The IC 50 values calculated from a partial least-squares (PLS) analysis model correlated well with experimental data ( R (2) = 0.99). Isoflavones and beta-conglycinin positively contributed to the cytotoxicity of soy on L1210 leukemia cells. Lunasin and BBI were potent L1210 cell inhibitors (IC 50 = 13.9 and 22.5 microM, respectively), but made modest contributions to the activity of defatted soy flour hydrolysates due to their relatively low concentrations. In conclusion, the data demonstrated that beta-conglycinins are among the major protein components that inhibit leukemia cell growth in vitro. Furthermore, it was feasible to differentiate soybean varieties on the basis of the biological effect of their components using a statistical model and a cell-based assay.
Wenyi Wang; Neal A Bringe; Mark A Berhow; Elvira Gonzalez de Mejia
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-05-13
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-04     Completed Date:  2008-07-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4012-20     Citation Subset:  IM    
Department of Food Science and Human Nutrititon, University of Illinois at Urbana-Champaign, Urbana-Champaign, IL 61801, USA.
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MeSH Terms
Antigens, Plant
Antineoplastic Agents, Phytogenic / pharmacology*
Cell Death / drug effects
Cell Line, Tumor
Globulins / analysis,  pharmacology*
Isoflavones / pharmacology
Leukemia L1210
Saponins / pharmacology
Seed Storage Proteins
Soybean Proteins / analysis,  chemistry*,  metabolism,  pharmacology
Soybeans / genetics
Species Specificity
Reg. No./Substance:
0/Antigens, Plant; 0/Antineoplastic Agents, Phytogenic; 0/Globulins; 0/Isoflavones; 0/Saponins; 0/Seed Storage Proteins; 0/Soybean Proteins; 0/beta-conglycinin protein, Glycine max

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