Document Detail


beta-Catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis.
MedLine Citation:
PMID:  16100089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of cell adhesion in epithelia is a fundamental process governing morphogenesis in embryos and a key step in the progression of invasive cancers. Here, we have analysed the molecular pathways controlling the epithelial organisation of somites. Somites are mesodermal epithelial structures of vertebrate embryos that undergo several changes in cell adhesion during early embryonic life. We show that Wnt6 in the ectoderm overlaying the somites, but not Wnt1 in the neighbouring neural tube, is the most likely candidate molecule responsible for the maintenance of the epithelial structure of the dorsal compartment of the somite: the dermomyotome. We characterised the signalling pathway that mediates Wnt6 activity. Our experiments suggest that the Wnt receptor molecule Frizzled7 probably transduces the Wnt6 signal. Intracellularly, this leads to the activation of the beta-catenin/LEF1-dependent pathway. Finally, we demonstrate that the bHLH transcription factor paraxis, which was previously shown to be a major player in the epithelial organisation of somites, is a target of the beta-catenin signal. We conclude that beta-catenin activity, initiated by Wnt6 and mediated by paraxis, is required for the maintenance of the epithelial structure of somites.
Authors:
Claudia Linker; Cynthia Lesbros; Jérôme Gros; Laura W Burrus; Alan Rawls; Christophe Marcelle
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  132     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-15     Completed Date:  2005-11-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3895-905     Citation Subset:  IM    
Affiliation:
Laboratoire de Génétique et de Physiologie du Développement (LGPD (IBDM), CNRS UMR 6545. Université de la Méditerranée, Campus de Luminy, case 907, 13288 Marseille, Cedex 09, France. c.linker@ucl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors
Chick Embryo
Cytoskeletal Proteins / metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Epithelium / embryology,  metabolism
Gene Expression Regulation, Developmental
Intercellular Signaling Peptides and Proteins / genetics,  metabolism*
Mice
Signal Transduction*
Somites / metabolism*
Trans-Activators / metabolism*
Wnt Proteins
Wnt1 Protein
Xenopus
Xenopus Proteins
beta Catenin
Grant Support
ID/Acronym/Agency:
1 R15 HD4204501/HD/NICHD NIH HHS; P20 MD000262/MD/NCMHD NIH HHS; S06 GM52588/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Catnb protein, mouse; 0/Cytoskeletal Proteins; 0/DNA-Binding Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Tcf15 protein, mouse; 0/Trans-Activators; 0/Wnt Proteins; 0/Wnt1 Protein; 0/Wnt1 protein, mouse; 0/Xenopus Proteins; 0/beta Catenin; 0/beta-catenin protein, Xenopus

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