| beta-Catenin is not necessary for maintenance or repair of the bronchiolar epithelium. | |
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MedLine Citation:
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PMID: 19213872 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Signaling by Wnt/beta-catenin regulates self-renewal of tissue stem cells in the gut and, when activated in the embryonic bronchiolar epithelium, leads to stem cell expansion. We have used transgenic and cell type-specific knockout strategies to determine roles for beta-catenin-regulated gene expression in normal maintenance and repair of the bronchiolar epithelium. Analysis of TOPGal transgene activity detected beta-catenin signaling in the steady-state and repairing bronchiolar epithelium. However, the broad distribution and phenotype of signaling cells precluded establishment of a clear role for beta-catenin in the normal or repairing state. Necessity of beta-catenin signaling was tested through Cre-mediated deletion of Catnb exons 2-6 in airway epithelial cells. Functional knockout of beta-catenin had no impact on expression of Clara cell differentiation markers, mitotic index, or sensitivity of these cells to the Clara cell-specific toxicant, naphthalene. Repair of the naphthalene-injured airway proceeded with establishment of focal regions of beta-catenin-null epithelium. The size of regenerative epithelial units, mitotic index, and restoration of the ciliated cell population did not vary between wild-type and genetically modified mice. Thus, beta-catenin was not necessary for maintenance or efficient repair of the bronchiolar epithelium. |
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Authors:
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Anna C Zemke; Roxana M Teisanu; Adam Giangreco; Jeff A Drake; Brian L Brockway; Susan D Reynolds; Barry R Stripp |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-02-12 |
Journal Detail:
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Title: American journal of respiratory cell and molecular biology Volume: 41 ISSN: 1535-4989 ISO Abbreviation: Am. J. Respir. Cell Mol. Biol. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-21 Completed Date: 2009-11-23 Revised Date: 2010-11-02 |
Medline Journal Info:
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Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 535-43 Citation Subset: IM |
Affiliation:
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Center for Lung Regeneration, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bronchioles / drug effects*, metabolism, pathology Cell Differentiation Cell Proliferation Gene Expression Regulation, Developmental Gene Knockout Techniques Integrases / genetics Lac Operon Mice Mice, Inbred C57BL Mice, Transgenic Mitotic Index Naphthalenes / toxicity Phenotype Promoter Regions, Genetic RNA, Messenger / metabolism Rats Regeneration* / drug effects, genetics Respiratory Mucosa / drug effects*, metabolism, pathology Signal Transduction* / drug effects, genetics Stem Cells / drug effects*, metabolism, pathology TCF Transcription Factors / genetics Time Factors Uteroglobin / genetics beta Catenin / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL064888/HL/NHLBI NIH HHS; R01 HL090146/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Catnb protein, mouse; 0/Clara cell secretory protein; 0/Naphthalenes; 0/RNA, Messenger; 0/TCF Transcription Factors; 0/Tcf7L2 transcription factor; 0/beta Catenin; 9060-09-7/Uteroglobin; 91-20-3/naphthalene; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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