| β-benzene hexachloride induces apoptosis of rat sertoli cells through generation of reactive oxygen species and activation of JNKs and FasL. | |
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MedLine Citation:
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PMID: 19760616 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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β-benzene hexachloride (β-BHC), the major metabolite of benzene-hexachloride (BHC), is a weak estrogen-like chemical. It is a known persistent organic pollutant and male reproductive toxicant. However, the mechanism by which β-BHC exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in β-BHC-induced toxicity in male reproductive system. The results indicated that β-BHC exposure at over 30 μM showed the induction of apoptotic cell death. β-BHC could induce elevation in reactive oxygen species (ROS) generation, increase in the leakage rate of LDH and MDA level, and decrease in SOD activity. In addition, there was an increase in the cellular levels of phospho-JNKs and FasL in the β-BHC-induced apoptosis; and a significant reduction of procaspase-3 and -8 was observed over 30-μM β-BHC treatment. The translocation of NF-κB enhanced with the increase of concentration of β-BHC. Furthermore, NAC administration, a scavenger of ROS, reversed β-BHC-induced apoptosis effects via inhibition of JNKs activation, FasL expression, and NF-κB translocation. These results lead us to speculate that ROS generation may play a critical role in the initiation of β-BHC-induced apoptosis by activation of the JNKs, translocation of NF-κB, expression of FasL, and further activation of caspase cascade. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 124-135, 2011. |
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Authors:
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Yuqin Shi; Yang Song; Yuping Wang; Yinan Wang; Xianmin Liang; Yafei Hu; Haige Yu; Xia Guan; Jin Cheng; Kedi Yang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Environmental toxicology Volume: 26 ISSN: 1522-7278 ISO Abbreviation: Environ. Toxicol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100885357 Medline TA: Environ Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 124-35 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Wiley Periodicals, Inc. |
Affiliation:
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MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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