| beta-Agonist stimulation produces changes in cardiac AMPK and coronary lumen LPL only during increased workload. | |
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MedLine Citation:
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PMID: 15687106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Given the importance of lipoprotein lipase (LPL) in cardiac and vascular pathology, the objective of the present study was to investigate whether the beta-agonist isoproterenol (Iso) influences cardiac LPL. Incubation of quiescent cardiomyocytes with Iso for 60 min had no effect on basal, intracellular, or heparin-releasable (HR)-LPL activity. Similarly, Iso did not change HR-LPL in Langendorff isolated hearts that do not beat against an afterload. In the intact animal, LPL activity at the vascular lumen increased significantly in the Iso-treated group, together with a substantial increase in rate-pressure product. This LPL increase was likely via mechanisms regulated by activation of AMP-activated protein kinase (AMPK) and inactivation of acetyl-CoA carboxylase (ACC280). In glucose-perfused hearts, simply switching from Langendorff to the isolated working heart (that beats against an afterload) induced increases in AMPK and ACC280 phosphorylation and enhanced HR-LPL activity. Provision of insulin and albumin-bound palmitic acid to the working heart was able to reverse these effects. In these hearts, introduction of Iso to the buffer perfusate duplicated the effects seen when this beta-agonist was given in vivo. Our data suggest that Iso can influence HR-LPL only during conditions of increased workload, mechanical performance and excessive energy expenditure, and likely in an AMPK-dependent manner. |
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Authors:
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Ding An; Girsh Kewalramani; Dake Qi; Thomas Pulinilkunnil; Sanjoy Ghosh; Ashraf Abrahani; Rich Wambolt; Michael Allard; Sheila M Innis; Brian Rodrigues |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-02-01 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 288 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-10 Completed Date: 2005-06-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1120-7 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, The Univ. of British Columbia, 2146 East Mall, Vancouver, BC, Canada V6T 1Z3. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AMP-Activated Protein Kinases Acetyl-CoA Carboxylase / metabolism Adrenergic beta-Agonists / pharmacology* Animals Blotting, Western Endothelium, Vascular / metabolism Heart / drug effects*, physiology Isoproterenol / pharmacology* Lipoprotein Lipase / genetics, metabolism* Male Multienzyme Complexes / metabolism* Myocardial Contraction / drug effects, physiology Myocardium / enzymology* Myocytes, Cardiac / enzymology Phosphorylation Physical Conditioning, Animal / physiology Protein-Serine-Threonine Kinases / metabolism* RNA / chemistry, genetics Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Stimulation, Chemical |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic beta-Agonists; 0/Multienzyme Complexes; 63231-63-0/RNA; 7683-59-2/Isoproterenol; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.1.1.34/Lipoprotein Lipase; EC 6.4.1.2/Acetyl-CoA Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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