Document Detail


The beneficial effect of L-cysteine supplementation on DNA oxidation induced by forced training.
MedLine Citation:
PMID:  16517176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Forced training is closely implicated with free radicals production and indication of tissue damage as well as DNA oxidation. AIM: To investigate the effect of L-cysteine (L-cys) supplementation on DNA oxidative damage found in basketball players after forced training. SUBJECTS AND METHODS: Blood was obtained from 10 players pre-game (group A), post-game (group B) and after 1 month L-cys (0.5 g 24 h(-1), orally) supplementation, pre- (group C) and post-training (group D). Total antioxidant status (TAS) and the biomarker of DNA oxidative damage 8-hydroxy-2-deoxyguanosine (8-OHdG) as well as creatine kinase (CK) and lactate dehydrogenase (LDH) were measured with commercial kits. RESULTS: TAS was increased in the groups with L-cys (group C and group D). Post-exercise 8-OHdG levels, CK and LDH were remarkably lower (0.16+/-0.03 ng ml(-1), 115+/-15 U l(-1), 417+/-90 U l(-1), respectively) in group D than those in group B (0.36+/-0.05 ng ml(-1), 286+/-12 U l(-1), 688+/-88U l(-1), p<0.001, respectively). 8-OH dG levels were negatively correlated with TAS(r=-0.718, p<0.01) and positively with CK levels(r=0.590, p<0.01). CONCLUSIONS: L-cys supplementation in basketball players may reduce DNA damage induced by training. The sulfur-containing amino acid may protect muscle cells "death" by increasing TAS and the cellular defense against oxidative stress.
Authors:
Stylianos Tsakiris; Theodore Parthimos; Nickolaos Parthimos; Theodore Tsakiris; Kleopatra H Schulpis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-03
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  53     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-17     Completed Date:  2006-06-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  386-90     Citation Subset:  IM    
Affiliation:
Department of Experimental Physiology, Medical School, University of Athens, P.O. Box 65257, GR-15401 Athens, Greece. stsakir@cc.uoa.gr
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Basketball
Creatine Kinase / blood
Cysteine / therapeutic use*
DNA / metabolism*
Deoxyguanosine / analogs & derivatives,  blood
Dietary Supplements
Free Radicals / metabolism
Humans
L-Lactate Dehydrogenase / blood
Male
Oxidation-Reduction
Physical Exertion / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/Free Radicals; 52-90-4/Cysteine; 9007-49-2/DNA; 961-07-9/Deoxyguanosine; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase

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