Document Detail


The behaviour of BHK cells and neutrophil leukocytes on collagen gels of defined mechanical strength.
MedLine Citation:
PMID:  2126224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study demonstrates how the mechanical strength of a series of collagen/composite gels can be measured using a penetrometer. It was found that the presence of fibrin in collagen gels resulted in increased gel strength. Similarly hyaluronic acid was found to increase the strength of collagen gels. Addition of heparin weakened collagen gels as did chondroitin-6-sulphate. Neutrophil migration into collagen gels was found to be inversely proportional to gel strength. Fibrin and hyaluronic acid containing gels inhibited neutrophil migration while the presence of heparin and chondroitin sulphate increased neutrophil migration. BHK gel contraction experiments demonstrated how the presence of fibrin prevents gel contraction. Despite increasing gel strength the presence of hyaluronic acid appeared to have no effect on BHK contraction of collagen gels. Similarly the presence of heparin or chondroitin sulphate had no effect on gel contraction by BHK cells.
Authors:
G G Reid; J M Lackie; S D Gorham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international reports     Volume:  14     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-03-08     Completed Date:  1991-03-08     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1033-45     Citation Subset:  IM; S    
Affiliation:
Department of Cell Biology, Glasgow University.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Movement
Cells, Cultured
Chondroitin Sulfates
Collagen
Cricetinae
Fibrin
Fibroblasts / cytology*
Gels
Hyaluronic Acid
Kidney
Mesocricetus
Neutrophils / cytology*
Stress, Mechanical
Chemical
Reg. No./Substance:
0/Gels; 9001-31-4/Fibrin; 9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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