Document Detail

The behavior of centrosomes in multinucleate cells formed after colcemid treatment.
MedLine Citation:
PMID:  2743998     Owner:  NLM     Status:  MEDLINE    
Multinucleate PtK1 cells were generated by treating the cells with colcemid for up to 60 h. Cells with scattered chromosomes reconstructed nuclear envelopes around these chromosomes. After recovery of up to 36 h these multinucleate cells went into mitosis. In such cells mainly two types of spindles are found: a bipolar spindle with some "accessory" half-spindles and multipolar mitotic apparatus with several equally-sized half-spindles ordered in an irregular way. Ultrastructural studies revealed centrosomes within such spindles which had not developed a microtubular connection to chromosomes and obviously could not act as mitotic pole. This result is interpreted in the way that centrosomes undergo a maturation cycle. Immature centrosomes cannot form mitotic poles. The asynchrony of the cycles of the multiple centrosomes seems to be generated by an uneven distribution of special factor(s).
R Dey; N Paweletz; S Ghosh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cell biology     Volume:  48     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-08-25     Completed Date:  1989-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  227-38     Citation Subset:  IM    
Department of Botany, University of Calcutta, India.
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MeSH Terms
Cell Nucleus / drug effects*,  ultrastructure
Cells, Cultured
Centromere / drug effects*,  ultrastructure
Chromosomes / drug effects*
Demecolcine / pharmacology*
Mitotic Spindle Apparatus / ultrastructure
Reg. No./Substance:

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