| bcl-2, bax, bcl-XL, and bcl-XS expression in normal and neoplastic ovarian tissues. | |
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MedLine Citation:
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PMID: 9516944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bcl-2 family of proteins includes some important regulators of apoptosis. Among these, bcl-2 and bcl-xL prevent cells from entering apoptosis, whereas bax and bcl-xS can induce cell death. Alterations in the control of this process can lead to a decrease in cell death, thus contributing to neoplastic growth. Diminished susceptibility to chemotherapy has also been attributed, in in vitro systems, to alterations in the levels of bcl-2, bax, or bcl-x. We analyzed the expression of bcl-2, bax, bcl-xL, and bcl-xS in normal and neoplastic ovarian tissues by reverse transcriptase-PCR and Western blotting. The RNA and protein levels were significantly correlated for all genes. Interestingly, the levels of these genes in normal and neoplastic tissues were significantly different: bcl-2 was higher in normal tissue (P < 0.002), whereas bax and bcl-xL were higher in carcinoma (P < 0.018 and P < 0.030, respectively). bcl-xS was present at low levels in 83% of neoplastic samples and was undetectable in normal tissue. Reverse transcriptase-PCR analysis of 74 tumors showed no major correlation with clinicopathological parameters or with response to chemotherapy. Only bax and bcl-xL were correlated with progesterone receptor levels (n = 29, r = +0.44, P < 0.0189, and r = -0.40, P < 0.035, respectively). No correlation was found with estrogen receptor levels or with p53 immunostaining. Our data indicate that the regulation of the bcl-2 family of proteins differs between normal and neoplastic ovarian tissues. Moreover, the modulation of these genes in ovarian carcinoma is different compared to other tissues; therefore, tissue specificity is very important in regulation of the bcl-2 family of proteins. |
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Authors:
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M Marone; G Scambia; S Mozzetti; G Ferrandina; S Iacovella; A De Pasqua; P Benedetti-Panici; S Mancuso |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical cancer research : an official journal of the American Association for Cancer Research Volume: 4 ISSN: 1078-0432 ISO Abbreviation: Clin. Cancer Res. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-04-09 Completed Date: 1998-04-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9502500 Medline TA: Clin Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 517-24 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Catholic University, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Female Humans Middle Aged Ovarian Neoplasms / metabolism* Ovary / metabolism* Proto-Oncogene Proteins c-bcl-2 / biosynthesis* RNA / metabolism RNA, Neoplasm / metabolism Reference Values bcl-X Protein |
| Chemical | |
Reg. No./Substance:
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0/BCL2L1 protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Neoplasm; 0/bcl-X Protein; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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