Document Detail

The basement membrane-type heparan sulfate proteoglycan (perlecan) in ameloblastomas: its intercellular localization in stellate reticulum-like foci and biosynthesis by tumor cells in culture.
MedLine Citation:
PMID:  12189507     Owner:  NLM     Status:  MEDLINE    
The localization and biosynthesis of basement membrane-type heparan sulfate proteoglycan (HSPG), known as perlecan, were studied in ameloblastomas using surgical tissue sections and cells in primary culture to demonstrate the existence of extracellular matrix (ECM) molecules in the intercellular space of epithelial tissue. HSPG was immunolocalized in the intercellular spaces of stellate reticulum-like cells and small vacuolar structures between basal cells in tumor cell nests as well as in myxofibrous stroma. By means of in-situ hybridization, mRNA signals for the HSPG core were intensely demonstrated in the cytoplasm of basal and parabasal cells of parenchyma. Furthermore, the in-vitro biosynthesis of HSPG core protein by ameloblastoma cells was confirmed using immunofluorescence, immunoprecipitation, and reverse-transcriptase polymerase chain reaction (RT-PCR). The results indicated that ameloblastoma cells synthesize HSPG and deposit it in their intercellular space. The intercellular HSPG might act as a carrier for transport of nutrients to tumor cells within ameloblastomatous foci.
Hiroko Ida-Yonemochi; Terué Ikarashi; Masaki Nagata; Hideyuki Hoshina; Ritsuo Takagi; Takashi Saku
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-11-07
Journal Detail:
Title:  Virchows Archiv : an international journal of pathology     Volume:  441     ISSN:  0945-6317     ISO Abbreviation:  Virchows Arch.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-21     Completed Date:  2002-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423843     Medline TA:  Virchows Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  165-73     Citation Subset:  IM    
Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Science, 2-5274 Gakkocho-dori, Niigata 951-8126, Japan.
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MeSH Terms
Ameloblastoma / genetics,  metabolism*,  pathology
Basement Membrane
Child, Preschool
Heparan Sulfate Proteoglycans / biosynthesis*,  genetics
Immunoenzyme Techniques
In Situ Hybridization
Jaw Neoplasms / genetics,  metabolism*,  pathology
RNA, Messenger / metabolism
RNA, Neoplasm / analysis
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells / metabolism,  pathology
Tumor Cells, Cultured / metabolism*,  pathology
Reg. No./Substance:
0/Heparan Sulfate Proteoglycans; 0/RNA, Messenger; 0/RNA, Neoplasm; 143972-95-6/perlecan

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