| UPLC/MS based method for quantitative determination of fatty acid composition in Gram-negative and Gram-positive bacteria. | |
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MedLine Citation:
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PMID: 20621131 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Quantitative fatty acid composition of microorganisms at various growth space points is required for understanding membrane associated processes of cells, but the majority of the relevant publications still restrict to the relative compositions. In the current study, a simple and reliable method for quantitative measurement of fatty acid content in bacterial biomass without prior derivatization using ultra performance liquid chromatography-electrospray ionization mass spectrometry was developed. The method was applied for investigating the influence of specific growth rate and pH on the fatty acid profiles of two biotechnologically important microorganisms - Gram-negative bacteria Escherichia coli and Gram-positive bacteria Lactococcus lactis grown in controlled physiological states. It was found that the membranes of slowly growing cells are more rigid and that the fatty acid fraction of the cells of L. lactis diminishes considerably with increasing growth rate. |
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Authors:
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Merli Spitsmeister; Kaarel Adamberg; Raivo Vilu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-16 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 82 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 288-95 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Competence Centre of Food and Fermentation Technologies, Akadeemia tee 15, 12618, Tallinn, Estonia. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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