Document Detail

A basal-defined medium for the study of proteolytic activity of Serratia marcescens.
MedLine Citation:
PMID:  10349724     Owner:  NLM     Status:  MEDLINE    
A simple defined basal medium is presented for the study of proteolytic activity, induction and repression, and protease purification with Serratia marcescens. Since the medium contains no protein, it does not interfere with or present artifact to protein assays, column chromatography, or electrophoresis. The medium consists of the basal salts and buffer medium of Bromke and Hammel (1979) plus a carbon-energy source such as glycerol, calcium chloride for the cation requirement for protease activity, and an amino acid, preferably leucine. Growth parameters and proteolytic activities are presented for unsupplemented medium and for the medium supplemented with each of 18 amino acids. Unsupplemented medium completes the logarithmic phase in 12.5 h of incubation and has a constitutive level of proteolytic activity. Supplementation with any amino acid, except cysteine and tryptophan, increases significantly the proteolytic activity, but has a varied effect on growth parameters.
B J Bromke; E Venuti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  45     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-07-14     Completed Date:  1999-07-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  88-91     Citation Subset:  IM    
Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, PA 19131, USA.
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MeSH Terms
Amino Acids / metabolism
Culture Media / chemistry*
Leucine / metabolism
Peptide Hydrolases / metabolism*
Serratia Infections / microbiology
Serratia marcescens / enzymology*,  growth & development*
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 61-90-5/Leucine; EC 3.4.-/Peptide Hydrolases

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