Document Detail


A bacteriophage of Bacillus subtilis which forms plaques only at temperatures above 50 C. I. Physical and chemical characteristics of TSP-1.
MedLine Citation:
PMID:  4112068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriophage TSP-1 was isolated from soil in a search for phage which would form plaques on Bacillus subtilis W168 at 53 C. It forms clear plaques only at temperatures from 50 to 55 C. Approximately 95% of the free phage adsorb after 2 min at 53 C. The lytic cycle is between 55 and 60 min long with a burst size of about 55 particles per infected bacterium. The phage was shown to contain double-stranded deoxyribonucleic acid with a base composition of 44.7% guanine plus cytosine. This deoxyribonucleic acid does not contain a base analogue for thymine and has a molecular weight estimated at 56 x 10(6) daltons.
Authors:
J R LaMontagne; W C McDonald
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  9     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1972 Apr 
Date Detail:
Created Date:  1972-06-29     Completed Date:  1972-06-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  646-51     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Bacillus subtilis*
Bacteriolysis
Bacteriophages / analysis,  growth & development*,  isolation & purification
Centrifugation, Density Gradient
Cesium
Chlorides
Chromatography, Paper
Cytosine / analysis
DNA, Viral / analysis
Guanine / analysis
Hot Temperature
Microscopy, Electron
Molecular Weight
Phosphorus Isotopes
Soil Microbiology
Spectrophotometry
Staining and Labeling
Time Factors
Viral Proteins
Virus Cultivation
Virus Replication
Chemical
Reg. No./Substance:
0/Chlorides; 0/DNA, Viral; 0/Phosphorus Isotopes; 0/Viral Proteins; 71-30-7/Cytosine; 73-40-5/Guanine; 7440-46-2/Cesium
Comments/Corrections

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