Document Detail


The bacA gene, which determines bacitracin susceptibility in Streptococcus pneumoniae and Staphylococcus aureus, is also required for virulence.
MedLine Citation:
PMID:  10878119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Homologues of Escherichia coli bacA, encoding extremely hydrophobic proteins, were identified in the genomes of Staphylococcus aureus and Streptococcus pneumoniae. Allelic replacement mutagenesis demonstrated that the gene is not essential for in vitro growth in either organism, and the mutants showed no significant changes in growth rate or morphology. The Staph. aureus bacA mutant showed slightly reduced virulence in a mouse model of infection and an eightfold increase in bacitracin susceptibility. However, a Strep. pneumoniae bacA mutant was highly attenuated in a mouse model of infection, and demonstrated an increase in susceptibility to bacitracin of up to 160000-fold. These observations are consistent with the previously proposed role of BacA protein as undecaprenol kinase.
Authors:
A F Chalker; K A Ingraham; R D Lunsford; A P Bryant; J Bryant; N G Wallis; J P Broskey; S C Pearson; D J Holmes
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  146 ( Pt 7)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-30     Completed Date:  2000-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1547-53     Citation Subset:  IM    
Affiliation:
Anti-Infective Research (UP1345), SmithKline Beecham Pharmaceuticals, 1250 South Collegeville Road, PO Box 5089, Collegeville, PA 19426-0989,USA. Alison_F_Chalker@sbphrd.com
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF228662
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Anti-Bacterial Agents / pharmacology
Bacitracin / pharmacology
Bacterial Proteins / chemistry,  genetics*,  physiology
Disease Models, Animal
Female
Male
Membrane Proteins / chemistry,  genetics*,  physiology
Membrane Transport Proteins*
Mice
Mice, Inbred CBA
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation
Phosphotransferases (Alcohol Group Acceptor) / genetics,  physiology
Pneumococcal Infections / microbiology
Sequence Homology, Amino Acid
Staphylococcus aureus / drug effects,  genetics*,  pathogenicity
Streptococcus pneumoniae / drug effects,  genetics*,  pathogenicity
Virulence
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Membrane Proteins; 0/Membrane Transport Proteins; 0/bacA protein, Rhizobium meliloti; 1405-87-4/Bacitracin; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.66/undecaprenol kinase

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