Document Detail


An autoradiographic method of detecting A. laidlawii and M. hyorhinis in cell cultures.
MedLine Citation:
PMID:  6173306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The autoradiographic investigation of L cells and chinese hamster cells for the presence of mycoplasmas (A. laidlawii and M. hyorhinis) using uridine/uracil (UdR/U) testing is a rapid and reliable method suitable for the serial checking of even a small number of cells. It depends on a reduced incorporation of [3H]uridine and an increased uptake of [3H]uracil into the RNA of mycoplasma-infected cells, shown in autoradiograms by the density of the grains and their distribution. Results obtained by the autoradiographic technique correspond approximately to specific activity values of RNA-infected cells after the incorporation of [3H]uridine and [3H]uracil.
Authors:
J Keprtova; K Jurmanova; V Spurna; E Minarova; J Hofmanova; M Nebola
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro     Volume:  17     ISSN:  0073-5655     ISO Abbreviation:  In Vitro     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1982-04-12     Completed Date:  1982-04-12     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0063733     Medline TA:  In Vitro     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  563-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography / methods*
Cell Line
Cricetinae
Cricetulus
L Cells (Cell Line) / microbiology
Lung
Mice
Mycoplasma / isolation & purification*
RNA / metabolism
Chemical
Reg. No./Substance:
63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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