Document Detail


The automation of cytochemical methods for automated cytophotometers.
MedLine Citation:
PMID:  70461     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of an automated differential white blood cell counter is reviewed. After the red cells have been lysed, the white cells are counted by staining and passing through an electro-optical chamber in liquid suspension, surrounded by a laminar, or sheath, stream. Staining procedures were made specific for each type of leukocyte, and separate channels were used for counting each type. Staining intensity and characteristics of the various types of blood cells are discussed. They relate to enzyme levels and the effect on differentiation and identification of the cells. Since reasons for some of the design features are not obvious, discussion of the relevant problems is included. Several applications that go beyond routine differential counting are described.
Authors:
A M Saunders
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1001-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Basophils
Histocytochemistry / methods*
Humans
Leukocyte Count / instrumentation,  methods*
Monocytes
Peroxidase
Photometry / instrumentation*
Staining and Labeling
Chemical
Reg. No./Substance:
EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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