Document Detail


An automated continuous-monitoring procedure for the determination of acid phosphatase activity in serum.
MedLine Citation:
PMID:  19173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The method of Hillmann, in which hydrolysis of alpha-naphthyl phosphate by acid phosphatase is coupled to the formation of an alpha-naphthol-Fast Red TR azo-compound, has been adapted for use with the LKB Produkter AB 8600 reaction rate analyzer. Factors which affect the reproducibility of the method are described and its performance is shown to be superior to that of a manual phenyl-phosphate procedure.
Authors:
R J Warren; D W Moss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  77     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1977 Jun 
Date Detail:
Created Date:  1977-10-14     Completed Date:  1977-10-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  179-88     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / blood*
Autoanalysis
Evaluation Studies as Topic
Female
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Kinetics
Male
Osteitis Deformans / enzymology
Prostatic Neoplasms / enzymology
Spectrophotometry / methods
Chemical
Reg. No./Substance:
0/Indicators and Reagents; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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