Document Detail


The automated classification of mitotic phase for human chromosome spreads.
MedLine Citation:
PMID:  330725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A system has been developed that automatically recognizes the mitotic phase of human chromosome spreads for karyotyping. Suitable spreads are classified into one of five subphases of mitosis. Classification is performed on the basis of summed chromosome length and most probable chromosome width. Classification requires 100-500 msec. A television camera scans the spread through microscope optics; computer and special purpose electronics process the video signals to generate run length histograms. The histograms are used to determine mitotic phase. Unbanded spreads, 133, were classified with a 4.5% error rate. One hundred banded spreads were classified with a 15% error rate.
Authors:
R P Bishop; I T Young
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  730-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Autoanalysis
Chromatids
Chromosomes*
Computers
Cytological Techniques*
Humans
Mitosis*
Television

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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