| The autologous serum skin test: a screening test for autoantibodies in chronic idiopathic urticaria. | |
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MedLine Citation:
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PMID: 10233264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One-third of patients with chronic idiopathic urticaria (CIU) have circulating functional autoantibodies against the high affinity IgE receptor FcepsilonRI, or IgE. The intradermal injection of autologous serum causes a weal and flare reaction in many patients with CIU, and this reaction forms the basis of the autologous serum skin test (ASST). We have determined the parameters of the ASST which define the optimal sensitivity and specificity for the identification of patients with autoantibodies. Two physicians (R.A. S. and C.E.H.G.) performed ASSTs in a total of 155 patients with CIU, 40 healthy control subjects, 15 patients with dermographism, nine with cholinergic urticaria and 10 with atopic eczema. Patients were classified as having functional autoantibodies by demonstrating in vitro serum-evoked histamine release from the basophils of two healthy donors. There were significant differences (P < 0.001) in the mean weal diameter, weal volume, weal redness and flare area of the intradermal serum-induced cutaneous responses at 30 min between patients with CIU with autoantibodies and either those without autoantibodies or control subjects. The optimum combined sensitivity and specificity of the ASST was obtained if a positive test was defined as a red serum-induced weal with a diameter of >/= 1.5 mm than the saline-induced response at 30 min. For R.A.S. and C.E.H.G., the ASST sensitivity was 65% and 71% and specificity was 81% and 78%, respectively. Using these criteria, the following subjects had positive ASSTs: none of 15 dermographics, none of 10 atopics, one of nine cholinergics and one of 40 controls. |
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Authors:
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R A Sabroe; C E Grattan; D M Francis; R M Barr; A Kobza Black; M W Greaves |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The British journal of dermatology Volume: 140 ISSN: 0007-0963 ISO Abbreviation: Br. J. Dermatol. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 2000-03-27 Completed Date: 2000-03-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0004041 Medline TA: Br J Dermatol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 446-52 Citation Subset: IM |
Affiliation:
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Professorial Unit, St John's Institute of Dermatology, UMDS, St Thomas' Hospital, London SE1 7EH, U.K. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Autoantibodies
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analysis* Chronic Disease Humans Immunoglobulin E / immunology Receptors, IgE / immunology Sensitivity and Specificity Skin Tests / methods Urticaria / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Receptors, IgE; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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