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The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG.
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MedLine Citation:
PMID:  3097237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rheumatic disease autoantigen, Ro/SSA, was immunogenic to a rabbit host. The heteroimmune rabbit serum bound the Ro/SSA particle in immunoblots and in an ELISA. Both the rabbit anti-Ro/SSA and a human prototype anti-Ro/SSA serum also bound IgG; and moreover, IgG inhibited both rabbit and human anti-Ro/SSA activity. Anti-IgG activity of the rabbit and human anti-Ro/SSA sera bound Ro/SSA by Western blot and solid-phase assays. In addition, purified Ro/SSA inhibited the anti-IgG activity of the anti-Ro/SSA sera from rabbit and man. Affinity purification of the IgG- and Ro/SSA-binding fractions of the rabbit anti-Ro/SSA demonstrated that both the anti-Ro/SSA and anti-IgG activities were concentrated in these fractions. These data show that Ro/SSA and IgG share epitopes that are bound by anti-Ro/SSA antibody. Inhibition experiments suggest that this antibody is found in most human anti-Ro/SSA autoimmune sera and that the epitope(s) are found in the F(ab')2 fragment of IgG.
Authors:
M J Mamula; O F Fox; H Yamagata; J B Harley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  164     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-08     Completed Date:  1987-01-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1889-901     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantigens / immunology*
Cattle
Enzyme-Linked Immunosorbent Assay
Humans
Immune Sera
Immunodiffusion
Immunoglobulin Fab Fragments / metabolism
Immunoglobulin G / immunology*
RNA, Small Cytoplasmic*
Ribonucleoproteins*
Grant Support
ID/Acronym/Agency:
AM-31133/AM/NIADDK NIH HHS; AM-34159/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Immune Sera; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G; 0/RNA, Small Cytoplasmic; 0/Ribonucleoproteins; 0/SS-A antigen; 0/TROVE2 protein, human
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 12 Year: 1986
Volume: 164 Issue: 6
First Page: 1889 Last Page: 1901
ID: 2188496
Publisher Id: 87059642
PubMed Id: 3097237

The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG


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