| The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG. | |
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MedLine Citation:
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PMID: 3097237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rheumatic disease autoantigen, Ro/SSA, was immunogenic to a rabbit host. The heteroimmune rabbit serum bound the Ro/SSA particle in immunoblots and in an ELISA. Both the rabbit anti-Ro/SSA and a human prototype anti-Ro/SSA serum also bound IgG; and moreover, IgG inhibited both rabbit and human anti-Ro/SSA activity. Anti-IgG activity of the rabbit and human anti-Ro/SSA sera bound Ro/SSA by Western blot and solid-phase assays. In addition, purified Ro/SSA inhibited the anti-IgG activity of the anti-Ro/SSA sera from rabbit and man. Affinity purification of the IgG- and Ro/SSA-binding fractions of the rabbit anti-Ro/SSA demonstrated that both the anti-Ro/SSA and anti-IgG activities were concentrated in these fractions. These data show that Ro/SSA and IgG share epitopes that are bound by anti-Ro/SSA antibody. Inhibition experiments suggest that this antibody is found in most human anti-Ro/SSA autoimmune sera and that the epitope(s) are found in the F(ab')2 fragment of IgG. |
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Authors:
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M J Mamula; O F Fox; H Yamagata; J B Harley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 164 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1986 Dec |
Date Detail:
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Created Date: 1987-01-08 Completed Date: 1987-01-08 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1889-901 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantigens / immunology* Cattle Enzyme-Linked Immunosorbent Assay Humans Immune Sera Immunodiffusion Immunoglobulin Fab Fragments / metabolism Immunoglobulin G / immunology* RNA, Small Cytoplasmic* Ribonucleoproteins* |
| Grant Support | |
ID/Acronym/Agency:
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AM-31133/AM/NIADDK NIH HHS; AM-34159/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/Immune Sera; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G; 0/RNA, Small Cytoplasmic; 0/Ribonucleoproteins; 0/SS-A antigen; 0/TROVE2 protein, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 12 Year: 1986 Volume: 164 Issue: 6 First Page: 1889 Last Page: 1901 ID: 2188496 Publisher Id: 87059642 PubMed Id: 3097237 |
| The Ro/SSA autoantigen as an immunogen. Some anti-Ro/SSA antibody binds IgG | |
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