Document Detail

The attraction of Trypanosoma cruzi to vertebrate cells in vitro.
MedLine Citation:
PMID:  826623     Owner:  NLM     Status:  MEDLINE    
A video technic is described that permits a quantification of the degree of attraction of Trypanosoma cruzi trypomastigotes to vertebrate cells in vitro. Bovine embryo skeletal muscle cells (BESM), HeLa cells and Vero cells all attract a myotropic strain of T. cruzi trypomastigotes. BESM cells, however, are 2-fold more attractive to trypomastigotes than HeLa cells and 10-fold more attractive than Vero cells. Heat-inactivation of BESM cells abolishes their ability to respire and also to attract T. cruzi trypomastigotes. As there is no difference in the endogenous oxygen consumption between BESM, HeLa, and Vero cells, it is unlikely that differences in the attraction of trypomastigotes to the 3 cell types are due to variations in the magnitude of pO2 or pCO2 gradients in the milieu around the cells.
J A Dvorak; C L Howe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of protozoology     Volume:  23     ISSN:  0022-3921     ISO Abbreviation:  J. Protozool.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-02-24     Completed Date:  1977-02-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985197R     Medline TA:  J Protozool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  534-7     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured*
Hela Cells
Oxygen Consumption
Trypanosoma cruzi / physiology*

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