Document Detail


The attenuated sopB mutant of Salmonella enterica serovar Typhimurium has the same tissue distribution and host chemokine response as the wild type in bovine Peyer's patches.
MedLine Citation:
PMID:  14654296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella enterica serovar Typhimurium is an important cause of enteric infections in farm animals and it is one of the most frequent food borne infections worldwide. Serovar Typhimurium lacking the sopB gene is attenuated for induction of host inflammatory response and fluid accumulation into the intestinal lumen, which correlates with clinical diarrhea. SopB is an inositol phosphate phosphatase, but its exact role in the pathogenesis of salmonellosis is still unclear. We employed the bovine ileal ligated loop model to compare the tissue distribution of a sopB mutant and its wild type parent serovar Typhimurium. Sections of the Peyer's patches were histologically processed and immuno-stained for detection of serovar Typhimurium. In addition, samples were processed for transmission electron microscopy, and the profile of expression of host chemokine and cytokine responses was assessed. Ultrastructurally both strains had the same ability to invade intestinal epithelial cells. No differences were detected in the tissue distribution of the sopB mutant and the wild type organism and both strains elicited the same profile of chemokines and pro-inflammatory cytokines. In conclusion, our results indicate that the attenuation of the sopB mutant is associated with pathogenic mechanisms other than invasion and distribution in host intestinal tissues.
Authors:
Bruno P Reis; Shuping Zhang; Renée M Tsolis; Andreas J Bäumler; L Garry Adams; Renato L Santos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Veterinary microbiology     Volume:  97     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-05     Completed Date:  2004-03-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  269-77     Citation Subset:  IM    
Affiliation:
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / immunology*
Cattle
Cattle Diseases / immunology,  microbiology*
Chemokines / biosynthesis*
Colony Count, Microbial
Ileum / microbiology
Male
Microscopy, Electron / veterinary
Mutation
Peyer's Patches / immunology*,  microbiology*,  ultrastructure
Salmonella Infections, Animal / immunology,  microbiology*
Salmonella typhimurium / genetics*,  pathogenicity,  ultrastructure
Tissue Distribution
Virulence
Grant Support
ID/Acronym/Agency:
AI40124/AI/NIAID NIH HHS; AI44170/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Chemokines; 0/SopB protein, Bacteria; EC 3.4.-/SopB protein, Salmonella

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