Document Detail

An atomic force microscopy study of Galleria mellonella apolipophorin III effect on bacteria.
MedLine Citation:
PMID:  21453676     Owner:  NLM     Status:  MEDLINE    
Apolipophorin III (apoLp-III) is an abundant hemolymph protein involved in lipid transport and immune response in insects. As revealed by LIVE/DEAD staining, incubation of Gram-negative and Gram-positive bacteria in the presence of Galleria mellonella apoLp-III led to growth inhibition of selected bacteria. An atomic force microscopy (AFM) study of bacterial cells after apoLp-III treatment showed considerable alterations in the cell surface of Bacillus circulans, Klebsiella pneumoniae and Salmonella typhimurium. Our results clearly demonstrate that apoLp-III disturbed the proper structure of the bacterial cell surface. The alterations were dissimilar to those caused by cationic antimicrobial peptide, cecropin B, suggesting a different mode of action against bacteria. The present results indicate that AFM provides a powerful tool for studying the interactions of apoLp-III with microbial cells.
Agnieszka Zdybicka-Barabas; Barbara Januszanis; Pawel Mak; Małgorzata Cytryńska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-28
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1808     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-05-10     Completed Date:  2011-07-01     Revised Date:  2013-05-13    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1896-906     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, Poland [corrected].
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MeSH Terms
Apolipoproteins / isolation & purification,  pharmacology*
Gram-Negative Bacteria / drug effects*
Gram-Positive Bacteria / drug effects*
Microbial Sensitivity Tests
Microscopy, Atomic Force / methods*
Moths / chemistry*
Reg. No./Substance:
0/Apolipoproteins; 0/apolipophorin III
Erratum In:
Biochim Biophys Acta. 2012 Jul;1818(7):1801

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