| An atomic force microscopy study of Galleria mellonella apolipophorin III effect on bacteria. | |
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MedLine Citation:
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PMID: 21453676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apolipophorin III (apoLp-III) is an abundant hemolymph protein involved in lipid transport and immune response in insects. As revealed by LIVE/DEAD staining, incubation of Gram-negative and Gram-positive bacteria in the presence of Galleria mellonella apoLp-III led to growth inhibition of selected bacteria. An atomic force microscopy (AFM) study of bacterial cells after apoLp-III treatment showed considerable alterations in the cell surface of Bacillus circulans, Klebsiella pneumoniae and Salmonella typhimurium. Our results clearly demonstrate that apoLp-III disturbed the proper structure of the bacterial cell surface. The alterations were dissimilar to those caused by cationic antimicrobial peptide, cecropin B, suggesting a different mode of action against bacteria. The present results indicate that AFM provides a powerful tool for studying the interactions of apoLp-III with microbial cells. |
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Authors:
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Agnieszka Zdybicka-Barabas; Barbara Januszanis; Pawel Mak; Małgorzata Cytryńska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-28 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1808 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-05-10 Completed Date: 2011-07-01 Revised Date: 2013-05-13 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1896-906 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, Poland [corrected]. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins / isolation & purification, pharmacology* Gram-Negative Bacteria / drug effects* Gram-Positive Bacteria / drug effects* Microbial Sensitivity Tests Microscopy, Atomic Force / methods* Moths / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/apolipophorin III |
| Comments/Corrections | |
Erratum In:
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Biochim Biophys Acta. 2012 Jul;1818(7):1801 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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